Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3933929 LIPASE FROM RHIZOMUCOR MIEHEI
Sigma Aldrich Fine Chemicals Biosciences Myeloperoxidase from human leukocytes lyophilized powder, >=50 units/mg protein | 9003-99-0 | MFCD00131641 | 5UN
Myeloperoxidase from human leukocytes lyophilized powder, >=50 units/mg protein | 9003-99-0 | MFCD00131641 | 5UN
Cayman Chemical ANTIBODY KU-0058948 25mg
A PARP1 and PARP2 inhibitor (IC50s = 3.4 and 1.5 nM, respectively); selective for PARP1 over PARP3, PARP4, and tankyrase (IC50s = 40, 1,200, and >10,000 nM, respectively); selectively inhibits the survival of embryonic stem cells lacking Brca1 or Brca2 over embryonic stem cells expressing wild-type Brca1 or Brca2; decreases the survival and induces apoptosis in P39 and MUTZ-3 AML cells at 1 µM; potentiates MS-275-induced cytotoxicity in patient-derived AML cells at 5 nM; reduces survival in PTEN-deficient HCT116 cells but not those expressing wild-type PTEN or HCT116+/neo
Aldevron Dnase I, 70ug
70ug dispense of Dnase I. DNase I nonspecifically cleaves single- or double-stranded DNA, producing di-, tri- and oligonucleotide products. DNase I is free of detectable ribonuclease activity.
Apexbio Technology LLC EZ Cap Mito-EGFP Probe mRNA (m1Ψ) 5x1mg (1mg/mL)
Mito-EGFP mRNA encodes a fusion protein composed of a mitochondrial localization sequence fused with enhanced green fluorescent protein (EGFP) Through fusion of EGFP with a mitochondrial-targeting signal it allows real-time observation of mitochondrial dynamics in living cells aiding in revealing mitochondria s key roles in energy metabolism signal transduction and apoptosis Due to its high fluorescence efficiency and mitochondria-specific localization Mito-EGFP simplifies mitochondrial visualization facilitating a clearer understanding of mitochondrial function and behavior in cells EZ Cap Mito-EGFP Probe mRNA (m1 ) is provided at 1 mg/mL and is specifically optimized for mitochondria-specific expression of green fluorescent protein labeling This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo
New England Biolabs, Inc. T4 Polynucleotide Kinase (3' phosphatase minus) – 1000 units
T4 Polynucleotide Kinase catalyzes the transfer and exchange of P from the gamma position of ATP to the 5 -hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3'-monophosphates. This modified version exhibits full kinase activity with no 3' phosphatase activity.
Cayman Chemical LIpase PosItIve Control 50uL
An AChE inhibitor (IC50 0.7 nM) selective for AChE over BChE (IC50 6.82 UM) increases the secretion of APP in primary rat basal forebrain cells at 10 UM induces repetitive firing of electrically stimulated gastrocnemius muscle in anesthetized cats at 5 UG/animal inhibits tubocurarine- or decamethonium-induced paralysis in anesthetized cats at 3 UG/animal
United States Biological Corporation NUCLEASE P-1 100 UNITS
Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) from Penicillium citrinum, a zinc dependent glyco-enzyme consisting of 270 amino acid residues, hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. Nuclease P1 is capable of hydrolyzing single stranded DNA and RNA completely to the level of mononucleoside 5-monophosphates. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0. Activity: > 300U/mg protein using RNA substrate Unit Definition: One unit of nuclease activity is defined as the amount of enzyme that produces 1umole of acid soluble nucleotides from RNA based on E = 10,600 for RNA hydrolysates per minute at 37 �C, pH 5.3.