Modifying Enzymes
Apexbio Technology LLC Dyngo-4a 50mg. 1430213-30-1. MFCD24849400
Dyngo-4a 50mg. 1430213-30-1. MFCD24849400
Apexbio Technology LLC E.-COLI-DNA-LIGASE-1000U
E coli DNA ligase is an enzyme used extensively in molecular biology research particularly within the fields of genetic engineering and biotechnology Originating from the bacterium Escherichia coli this enzyme plays a crucial role in DNA replication and repair by catalyzing the formation of phosphodiester bonds between the 3 -hydroxyl and 5 -phosphate ends of DNA fragments Its mechanism of action involves the joining of Okazaki fragments on the lagging strand during DNA replication as well as sealing nicks in the DNA backbone It exhibits high activity in in vitro applications typically within the nanomolar to low micromolar range E coli DNA ligase is commonly used in applications involving cell models animal studies and drug screening protocols where its ability to ligate DNA fragments is essential Experimental concentrations typically depend on the specific research design allowing for flexibility in various experimental setups
Cayman Chemical 2-Oleoyl Glycerol 5mg
A natural monoacylglycerol that is metabolized by monoacylglycerol lipase; an agonist of GPR119 (EC50 = 2.5 µM), stimulating the release of glucagon-like peptide-1 from intestinal L-cells
Worthington Biochemical Corporation COLLAGENASE TYPE 2 FI 1000MG
Collagenase, Type 2 (Code: CLS-2), which is filtered through a 0.22 micron membrane and lyophilized in vials. 2593-92-3
Zymo Research Corporation ZymoTaq™ PreMix (50 Rxns)
The inclusion of a heat-activated, thermal-stable DNA polymerase reduces primer dimer and non-specific product formation that can occur when performing conventional PCR. This unique product is specifically designed for the amplification of bisulfite-treated DNA for methylation detection, realtime and quantitative PCR that are SYBR Green and probe based. The ZymoTaq™ PreMix yields specific amplicon formation with little or no byproducts. Simple and easy to use, just add water, primers, and template DNA to the ZymoTaq™ PreMix and then heat at 95oC for 10 minutes to initiate polymerization.ZymoTaq™ DNA polymerase is a heat-activated, hot start polymerase that has 3-terminal transferase activity. The addition of A overhangs to amplified DNA makes it ideal for use in TA-cloning.
Sigma Aldrich Fine Chemicals Biosciences Carbonic Anhydrase Isozyme II from bovine erythrocytes lyophilized powder, >=3,000 W-A units/mg protein | 9001-03-0 | MFCD00081467 | 25MG
Carbonic Anhydrase Isozyme II from bovine erythrocytes lyophilized powder, >=3,000 W-A units/mg protein | 9001-03-0 | MFCD00081467 | 25MG
ABclonal Technology Exonuclease I (E. coli)
Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA in the 3 to 5 direction, and does not degrade DNA strands with the 3-OH end blocked by phosphoryl or acetyl groups. Exonuclease I has strict substrate specificity and is incapable of degrading double-stranded DNA and RNA.
United States Biological Corporation NUCLEASE P-1 100 UNITS
Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) from Penicillium citrinum, a zinc dependent glyco-enzyme consisting of 270 amino acid residues, hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. Nuclease P1 is capable of hydrolyzing single stranded DNA and RNA completely to the level of mononucleoside 5-monophosphates. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0. Activity: > 300U/mg protein using RNA substrate Unit Definition: One unit of nuclease activity is defined as the amount of enzyme that produces 1umole of acid soluble nucleotides from RNA based on E = 10,600 for RNA hydrolysates per minute at 37 �C, pH 5.3.
ABclonal Technology Acetyl-CoA Carboxylase
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA the rate-limiting step in fatty acid synthesis There are two ACC forms alpha and beta encoded by two different genes ACC-alpha is highly enriched in lipogenic tissues The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA Multiple alternatively spliced transcript variants divergent in the 5 sequence and encoding distinct isoforms have been found for this gene
Cayman Chemical Oleoyl EthnolamIde 500mg
An analog of the endocannabinoid AEA found in brain tissue and in chocolate whose biosynthesis is reduced in the intestine of rats following food deprivation; an endogenous, potent agonist for PPARα (EC50 = 120 nM in a transactivation assay); systemic administration suppresses food intake and reduces weight gain in rats (10 mg/kg intraperitoneally) and PPARα wild type mice, but not in PPARα knockout mice
ABclonal Technology Exonuclease VII
Exonuclease VII (Exo VII) can cleave single-stranded DNA in both 5-3 and 3-5 directions and is inactive against linear or circular double-stranded DNA. When PCR requires stepwise completion to obtain the final target product, this enzyme can be used to remove residual oligonucleotide primers left during intermediate stages of PCR, eliminating interference with subsequent use of different primers. The enzymes digestion of single-stranded DNA is independent of metal ions. This product is a highly purified protein obtained through the recombinant expression of the Exonuclease VII gene (XseA and XseB).