Modifying Enzymes
INTACT GENOMICS INC Exonuclease III 10000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios (3-5).Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
INTACT GENOMICS INC T4 DNA Ligase 100,000 Units (400 units/µl)
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).Applications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Next-gen library preparation• Joining linkers and adapters to cohesive or blunt-ended DNA• Nick repair in duplex DNA, RNA or DNA/RNA hybrids• Self-circularization of linear DNA
Sigma Aldrich Fine Chemicals Biosciences Clara-Diastase mixture of enzymes, powder, >=35 U/mg | 9001-11-0 | MFCD00146469 | 10G
Clara-Diastase mixture of enzymes, powder, >=35 U/mg | 9001-11-0 | MFCD00146469 | 10G
Worthington Biochemical Corporation PURIFIED HYALURONIDASE 15KU
Chromatographically purified. A dialyzed, lyophilized powder.
AVIDITY LLC BIO-500 Biotin Solution, 5 mM d-biotin, 10 mL, 10 mM Bicine buffer, pH 8.3, Filter sterilized (0.22 μm), Room temperature shipping, -20 °C storage
The AVIDITY LLC BIO500 is a 5mM d-biotin solution supplied in a 10mL volume, designed for critical molecular biology applications. It is particularly suitable for in-vivo biotinylation culture inductions utilizing pBirAcm BirA over-expression plasmids and AviTag™-protein fusion vectors. The solution is also appropriate for large-scale BirA reactions requiring supplemental d-biotin and for supplementing induction growth media. Filter sterilized through a 0.22μm filter, this product ensures sterility for sensitive scientific protocols.
- Concentration: 5 mM d-biotin
- Volume: 10 mL
- Buffer: 10 mM Bicine
- pH: 8.3
- Sterility: Filter Sterilized (0.22 μm)
- Shipping Condition: Room temperature
- Storage Temperature: -20 °C
- Primary Application: In-vivo biotinylation
- Secondary Application: Large scale BirA reactions
Discovery Life Sciences HUMAN UGT1A1 0.5ML@5MG/ML
BD Gentest, Human UGT1A1, Regulatory Status is RUO, 0.5mL@5mg/mL
LGC GENOMICS LLC (Lucigen) CircLigase II ssDNA Ligase, 5,000 U @ 100 U/LAn ATP-independent, non-catalytic thermostable ligase that catalyses the intramolecular ligation (i.e. circularisation) of ssDNA and ssRNA templates.
CircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5-phosphate and a 3-hydroxyl group
Worthington Biochemical Corporation ENDOPROTEINASE GLU-C 1MG
Protease, S. Aureus (Endoproteinase Glu-C), Code: STAP, Minimum activity: 500U/mg dry weight, A lyophilized powder, Store at 2 deg. to 8 deg.C, 1mg
Sigma Aldrich Fine Chemicals Biosciences BENZONASE PURITY GRADE II
NC3761543 BENZONASE PURITY GRADE II
STEMCELL Technologies Proteinase K Solution, Size: 4 mL
Proteinase K is a serine protease that exhibits broad cleavage activity, cutting peptide bonds adjacent to the carboxylic group in aliphatic and aromatic amino acids. Proteinase K is used for a variety of applications, including removal of nucleases from DNA and RNA samples and terminating enzymatic reactions. Proteinase K Solution is in a ready-to-use format that is stable at room temperature and active over a pH range of 4.3 - 12.0 in 0.5% SDS or 1% Triton X-100. Product is free of nuclease activity. Proteinase K Solution is required for use with ArciTect™ T7 Endonuclease I Kit (Catalog #76021).
GENOVIS INC FabRICATOR LE, 5000 Units
FabRICATOR (IdeS) is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab')2 and Fc/2 fragments. No other substrate besides IgG is known.The IgG is digested within 30 minutes, and there is no risk of overdigestion if the incubation time is prolonged. Since FabRICATOR digests IgG under physiological reaction conditions, the immunoreactivity is preserved. FabRICATOR digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species, we recommend using FabRICATOR Z .
Cellscript Inc Cap-Clip™ Acid Pyrophosphatase | 100 units
For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer.
New England Biolabs, Inc. Template Switching RT Enzyme Mix – 20 reactions
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template. These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.
Enzo Life Sciences Matrix metalloproteinase (MMP) multipack-2 (1 Kit)
The MMP multipack-2 contains 10µg each of five highly active recombinant MMP catalytic domains: MMP-3, MMP-7, MMP-10, MMP-11, and MMP-12. Contents: Contains 10µg of each enzyme, provided in a screw-cap microfuge tube. MMP-3 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE109), MMP-7 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE181), MMP-10 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE392), MMP-11 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE282), MMP-12 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE138). Application: FUNC, HTS. Long Term Storage: -80°C
