Modifying Enzymes
XenoTech,LLC EASYCYP CYP2C9 3R+ 0.5 NMOL
EasyCyp CYP2C9*3 Reductase plus b5 0.5 nmol P450 at 1 nmol/ml, 10 mg/ml protein
Sigma Aldrich Fine Chemicals Biosciences alpha-Amylase from Aspergi50G
Microbial amylases are exoenzymes and are used in several industrial applications such as production of bread maltose syrups and fermentation of soya sauce miso etc. Amylase isolated from Aspergillus oryzae has been found to have molecular weights of 51kDa (sedimentation and diffusion) and 49kDa (gel filtration).
STEMCELL Technologies DNase I, ACF, Size: 25000 U
Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials and purified by chromatography. DNase I is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I, ACF preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium l (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
Abcam Dyngo 4a, Novel, highly potent dynamin inhibitor, 5MG
Dyngo 4a, Novel, highly potent dynamin inhibitor (IC50 values are 400 nM and 200 nM at dynamin I and II human-recombinant, respectively). Inhibits clathrin-mediated endocytosis (IC50 = 5.5 µM). Dynasore (ab120192) analog. Prevents botulin neurotoxin A (BoNTs) uptake and internalization, and prevents SNAP25 cleavage. Inhibits BoNTs-induced paralysis and delays botulism onset. - Cited in over 75 publications - Purity > 99% - Soluble in DMSO to 100 mM
The product is subject to the following: Abcam Restricted Use Statement
New England Biolabs, Inc. Thermostable USER III Enzyme - 50 units
Thermostable USER (Uracil-Specific Excision Reagent) III Enzyme generates a single nucleotide gap at the location of a uracil. Thermostable USER III Enzyme is a mixture of Afu Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease IV. Afu UDG catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease IV breaks the phosphodiester backbone at the 3’ and 5’ sides of the abasic site so that base-free deoxyribose is released leaving a 5’ deoxyribose phosphate (dRP) and a 3’-hydroxyl. Thermostable USER III Enzyme also has 3’-diesterase activity which can remove 3’ phosphate, 3’-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. Thermostable USER III Enzyme is optimally active at 65C.
New England Biolabs, Inc. NEBExpress T4 Lysozyme
NEBExpress T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the beta 1-4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria). It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites.
New England Biolabs, Inc. Thermostable OGG - 500 units
Thermostable OGG is an archaeal 8-oxoguanine (8oxoG) DNA glycosylase which acts both as aN-glycosylase and an AP-lyase. TheN-glycosylase activity releases the damaged purine (8-oxoguanine) from double stranded DNA, generating an apurinic (AP) site. The AP-lyase activity cleaves 3' to the AP site leaving a 5' phosphate and a 3'-phospho-alpha, beta-unsaturated aldehyde. Unlike some other DNA glycosylases, Thermostable OGG specifically recognizes and cleaves only 8oxoG and no other modified bases.
Amsbio LLC Chondroitinase ABC protease free (lyophilized) 2U/vial
Chondroitinase ABC protease free (lyophilized) 2U/vial
Medchemexpress LLC HY-19801 5mg Medchemexpress, CVT-10216 CAS:1005334-57-5 Purity:>98%
Medchemexpress, HY-19801 5mg CVT-10216 CAS:1005334-57-5 CVT-10216 is a highly selective, reversible aldehyde dehydrogenase-2 (ALDH-2) inhibitor with an IC50 of 29 nM. CVT-10216 also has inhibitory effect of ALDH-1 with an IC50 of 1.3 μM. CVT-10216 can reduce excessive alcohol drinking in alcohol-preferring rats and exhibit anxiolytic effects. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Sigma Aldrich Fine Chemicals Biosciences Lysoganglioside-GM1 from bovine brain >=95%, lyophilized powder | 171483-40-2 | MFCD00677269 | 1MG
Lysoganglioside-GM1 from bovine brain >=95%, lyophilized powder | Purity: >=95% | 171483-40-2 | MFCD00677269 | 1MG
Aldevron 2'O-Methyltransferase, 1450ug
1450ug dispense of 2'O-Methyltransferase. 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the m7Gppp5'N cap. 2'-O-Methyltransferase utilizes S-adenosylmethionine as the methyl group donor.
Aldevron SpyFi Cas9 Nuclease, 1mg
5mg dispense size of SpyFi Cas9 Nuclease. From basic research to clinical applications, our SpyFi Cas9 Nuclease delivers greater editing efficiency and reduced off-target effects without loss of on-target activity to help accelerate your CRISPR-Cas9 gene editing program. SpyFi Cas9 Nucleases are available at Research-Grade through Fisher, or across all quality grades (Research Grade or GMP) directly through Aldevron.
SpyFi Cas9 Nuclease is uniquely positioned to satisfy a wide range of needs from basic research to clinical applications. When used in combination with effective guide, target site and double-stranded DNA cut strategies, SpyFi Cas9 Nuclease delivers greater editing efficiency, higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Functional Performance
•Reduced off-target effects
•Ready to use for electroporation or transfection
•10 mg/mL formulation for ease of use
Aldevron Eureca-V Nuclease, 1mg
1mg dispense of Eureca-V Nuclease. CRISPR protein, Eureca-V™ Nuclease is the most recent addition to our catalog. It is based on the proprietary MAD7 nuclease developed by Inscripta. Aldevron and Inscripta have partnered to make this protein broadly available to the therapeutic, diagnostic, and agricultural research markets.Eureca-V is categorized as a Class 2, Type V CRISPR-Cas nuclease. It shares key features common to this family of CRISPR nucleases, including:
•Targets broad array of T-rich PAM domains (YTTV preferred, TTTN functions)
•Requires a single crRNA to cut target DNA sequence
•Staggered double-strand break at cut site creates 5’ overhangs
This product is subject to the following: Limited Use Label License
Aldevron Eureca-V Nuclease, 5mg
5mg dispense of Eureca-V Nuclease. CRISPR protein, Eureca-V™ Nuclease is the most recent addition to our catalog. It is based on the proprietary MAD7 nuclease developed by Inscripta. Aldevron and Inscripta have partnered to make this protein broadly available to the therapeutic, diagnostic, and agricultural research markets.Eureca-V is categorized as a Class 2, Type V CRISPR-Cas nuclease. It shares key features common to this family of CRISPR nucleases, including:
•Targets broad array of T-rich PAM domains (YTTV preferred, TTTN functions)
•Requires a single crRNA to cut target DNA sequence
•Staggered double-strand break at cut site creates 5’ overhangs
This product is subject to the following: Limited Use Label License