Modifying Enzymes
Cayman Chemical N-Oleoyl GlycIn 100mg
An endogenous lipoamino acid; increases triglyceride levels in 3T3-L1 adipocytes, an effect that can be reversed by SR141716; selectively induces the expression of mRNA encoding the CB1 receptor over mRNA encoding the CB2 receptor in the same cells at 50 µM; potentiates glycine-induced chloride currents in Xenopus oocytes expressing α1-, α1β-, α2-, α2β-, or α3 subunit-containing glycine receptors but not those expressing α3β subunit-containing glycine receptors at 1 µM; increases c-Fos protein levels, intracellular calcium levels, and AgRP secretion in mHypoE-38 cells, effects that can be reversed by AM251; reduces binge-like ethanol intake in alcohol-preferring mice without affecting total fluid intake and does not affect sucrose intake in the sucrose preference test in mice at 60 mg/kg; reduces balance impairments, thermal hyperalgesia, mechanical allodynia, aggressiveness, and immobility time in the tail suspension test in a mouse model of traumatic brain injury at 50 mg/kg
Cayman Chemical Oleoyl SerotonIn-d17 500UG
An internal standard for the quantification of oleoyl serotonin by GC- or LC-MS
Cayman Chemical Oleoyl SerotonIn-d17 100UG
An internal standard for the quantification of oleoyl serotonin by GC- or LC-MS
New England Biolabs, Inc. Template Switching RT Enzyme Mix – 20 reactions
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template. These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.
Enzo Life Sciences Ubiquitin activating enzyme E1 (human), (recombinant) (His-tag) (50 µg)
Essential ubiquitinylation pathway enzyme. MW: 118 kDa. Purity: ≥90% (SDS-PAGE). Formulation: In 50mM TRIS-hydrochloric acid, containing 1.0mM dithiothreitol. Source: Produced in E. coli BL21 (λDE3) expression system and containing a C-terminal His6-tag. Full length human ubiquitin-activating enzyme E1. UniProt: P22314. Handling: Avoid freeze/thaw cycles. Long Term Storage: -80°C
Worthington Biochemical Corporation DEOXYRIBONUCLEASE 1
Recombinant Bovine pancreatic deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases & proteases. A liquid preparation in 5mM Calcium Acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.
Discovery Life Sciences 1ULTRA CYTOSOL NEG CONTROL 5MG
Corning Supersomes Ultra Cytosol Negative Control, 5 mg/mL, 0.5 mL
Sigma Aldrich Fine Chemicals Biosciences Chymotrypsin United States Pharmacopeia (USP) Reference Standard | 9004-07-3 | 300MG
Chymotrypsin United States Pharmacopeia (USP) Reference Standard | 9004-07-3 | 300MG
Carnabio Usa Inc WNK4/10UG/CATALYTIC
WNK4, Catalytic domain, Wild type, Amino Acid 1-444, NP_115763.2, Expressed in Insect (sf21); 10microg
New England Biolabs, Inc. Immobilized T4 DNA Ligase - 1.1 mg
Immobilized T4 DNA Ligase (IM-T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, enzyme can be removed using a magnet thereby avoiding heat inactivation, and can be re-used.
New England Biolabs, Inc. EnGen Lba Cas12a (Cpf1) – 2000 pmol
EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) . Targeting requires a gRNA complementary to the target site as well as a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends. EnGen Lba Cas12a has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
New England Biolabs, Inc. Nucleoside Digestion Mix – 50 reactions
The Nucleoside Digestion Mix is a mixture of enzymes that provides a convenient one-step method to generate single nucleosides from DNA or RNA. Optimized for quantitative analysis by liquid chromatography-mass spectrometry (LC-MS), this reagent eliminates the need for sequential multi-step, time-consuming digestion protocols. The Nucleoside Digestion Mix digests ssDNA, dsDNA, DNA/RNA hybrids and RNA (except mRNA cap structures) containing epigenetically modified (m5C, hm5C, f5C, ca5C, m4C, m6A, etc.), unnatural, or damaged bases. Moreover, the low-glycerol formulation (<1%) significantly reduces glycerol-induced ion suppression during mass spectrometry analysis.
New England Biolabs, Inc. α1-2,4,6 Fucosidase O - 400 units
a1-2,4,6 Fucosidase O is a broad specificity exoglycosidase that catalyzes the hydrolysis of terminal a1-2, a1-4 and a1-6 linked fucose residues from oligosaccharides. a1-2,4,6 Fucosidase O cleaves a1-6 fucose residues more efficiently than other linkages.
New England Biolabs, Inc. yDcpS – 4000 units
yDcpS is the scavenging decapping enzyme from S. cerevisiae that hydrolyzes the phosphodiester bond between the gamma and beta phosphates of m7G-capped mRNA, producing diphosphorylated 5 ends and m7GMP. yDcpS is capable of decapping mRNAs of various lengths, decaps Cap0 and Cap1 with equal efficiency, and the diphosphorylated 5 end it produces is suitable for recapping with Vaccinia Capping Enzyme.