Modifying Enzymes
New England Biolabs, Inc. EnGen Lba Cas12a (Cpf1) – 70 pmol
EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) . Targeting requires a gRNA complementary to the target site as well as a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends. EnGen Lba Cas12a has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
New England Biolabs, Inc. EpiMark Methylated DNA Enrichment Kit – 25 reactions
The EpiMark Methylated DNA Enrichment Kit will selectively bind and enrich double-stranded methyl-CpG DNA from fragmented genomic DNA with as little as 5 ng of input DNA. Methylated DNA is isolated from fragmented genomic DNA (5 ng-25 ug) by binding to the methyl-CpG binding domain of human MBD2 protein fused to the Fc tail of human IgG1 (MBD2-Fc), which is coupled to paramagnetic hydrophilic protein A beads (MBC2-Fc/Protein A Magnetic Bead). Two Fc domains can be bound to one site on protein A with high affinity (Kd=10-7). As the Fc fragment is a dimer, four MBD2 domains are exposed to the solvent per molecule of protein A, increasing the relative equilibrium constant 100-fold. This stable complex will selectively bind double-stranded methylated CpG containing DNA. After simple wash steps followed by magnetic capture, the enriched DNA sample is easily eluted in a small volume of nuclease-free water by incubation at 65C.
Worthington Biochemical Corporation DIAPHORASE 1 KU
Diaphorase, Code: DILW, Store at 2 deg. to 8 deg.C, Minimum activity: 30U/mg dry weight, Supplied as a dialyzed, lyophilized powder, Unit Definition: 1 Unit equals a decrease in absorbance at 600nm of 1.0 per minute at 25 deg.C, pH 7.5, 1kU
Agilent Technologies GLYKO SIALIDASE ASAMPLER KIT
Try our lower MW isoforms for challenging sialidase digests.This Sampler Kit contains 1 vial each of 3 isoforms (88, 66 and 51 kDa) of the enzyme Sialidase A. Lower MW sialidases may help desialylate glycans if steric hindrance is an issue with regular sialidase.
Vector Biolabs Deoxyribonuclease I Adenovirus (Ad-DNASE I)
Ready to use in vivo grade AAV and Adenovirus Ready to use in vivo grade AAV and Adenovirus pre synthesized DNA vector High titer viral vectors 1/EA
Biotium Double Stranded DNA (dsDNA)(AE-2), CF488A conjugate, 0.1mg/mL
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.,Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds be
AnaSpec ADAMTS-4/Aggrecanase FRET Substrate, FRET Assay, 1 mg
The ADAMTS-4/Aggrecanase FRET Substrate offers researchers a reliable tool for detecting ADAMTS-4 enzyme activity. With a quantity of 1 mg, this peptide substrate enables precise enzyme assays with FRET technology, distinguishing it from standard substrates. Its specific function in cartilage degradation pathways makes it vital for studies related to osteoarthritis and tissue remodeling. Easy to use and store, it supports ongoing research in enzyme substrates and inhibitors.
Agilent Technologies CLONED PFU DNA POLYM
Cloned Pfu DNA Polymerase is a recombinant version for high-fidelity PCR and primer-extension reactions. Utilized for PCR cloning and generating blunt-end amplification product. Cloned Pfu DNA polymerase, 10X Cloned Pfu buffer.
New England Biolabs, Inc. NcoI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence C^CATG_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. DNA Polymerase I, Large (Klenow) Fragment – 1000 units
DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3' to 5' exonuclease activity, but has lost 5' to 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
New England Biolabs, Inc. RNase I – 25000 units
Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5'hydroxyl and 2',3' cyclic monophosphate. It has a preference for single-stranded RNA over double-stranded RNA. RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.
Haematologic Technologies Inc HUMAN PLASMIN 500UG
Coagulation protein, Human Plasmin, 500 micrograms, Native enzyme, Mode of action: Enzyme involved in fibrinolysis, Research use only, 50% (v/v) glycerol/water, store at -20 Celsius.