This mouse monoclonal antibody provides highly specific detection of digoxigenin, a widely used steroid hapten incorporated into nucleic acid probes for hybridization‑based assays. Clone 100 delivers strong affinity and low background, supporting sensitive detection in ELISA, Western blotting, in situ hybridization, and probe‑labeling workflows. Its clean, reproducible signal enables accurate measurement of DIG‑labeled DNA or RNA in complex matrices, making it ideal for molecular biology, assay development, and nucleic acid quantification. MDIG‑65A‑100 offers consistent lot‑to‑lot performance, ensuring reliable probe detection across diverse research applications.