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This module is part of the non-directional Ultra II RNA workflow, for Illumina- compatible non-strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.
Designed for use in the NEBNext Ultra II non-directional RNA workflow
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MicroRNA Inhibitor Negative Control is a full-length nucleotide 2 -methoxy modified oligonucleotide and can be used as a negative control The sequence of MicroRNA Inhibitor Negative Control is derived from cel-mir-239b It has minimal sequence identity with miRNAs in human mouse and rat
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hsa-miR-150-5p inhibitors are chemically-modified oligonucleotides that hybridize with mature miRNAs The miRNA inhibitors have full-length nucleotide 2 -methoxy modification The miRNA inhibitors strongly compete with mature miRNAs to prevent the complementary pairing of miRNAs and their target genes thereby inhibiting miRNAs from functioning
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