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This module is part of the non-directional Ultra II RNA workflow, for Illumina- compatible non-strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.
Designed for use in the NEBNext Ultra II non-directional RNA workflow
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PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA.It can discriminate between perfectly matched and non-matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA-RNA duplex.This enzymatic function is reliant upon the presence of Mg2+ ions, with a recommended concentration of 5 mM for most applications.PureRec DSN is overexpressed and extensively purified from a non-animal source and is free of non-specific nucleases or RNases.
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hsa-miR-17-5p inhibitors are chemically-modified oligonucleotides that hybridize with mature miRNAs The miRNA inhibitors have full-length nucleotide 2 -methoxy modification The miRNA inhibitors strongly compete with mature miRNAs to prevent the complementary pairing of miRNAs and their target genes thereby inhibiting miRNAs from functioning
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hsa-miR-204-5p inhibitors are chemically-modified oligonucleotides that hybridize with mature miRNAs The miRNA inhibitors have full-length nucleotide 2 -methoxy modification The miRNA inhibitors strongly compete with mature miRNAs to prevent the complementary pairing of miRNAs and their target genes thereby inhibiting miRNAs from functioning
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hsa-miR-21-5p inhibitors are chemically-modified oligonucleotides that hybridize with mature miRNAs The miRNA inhibitors have full-length nucleotide 2 -methoxy modification The miRNA inhibitors strongly compete with mature miRNAs to prevent the complementary pairing of miRNAs and their target genes thereby inhibiting miRNAs from functioning
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More