DNA Vectors
Creative Biomart Recombinant Protein 28.8 kDa Host-E.coli Inquiry 50 UG 1/EA
mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability The recombinant mCherry is expressed and purified from transformed E coli using a method that ensures high purity and maximal fluorescence intensity The protein is a 28 8 kDa monomer with 256 amino acids pI 6 23 Ex 587 nm (540-590 nm) Em 610 nm (550-650 nm) The protein is engineered with 6xHis-tag on the N-terminus
Vector Biolabs Enhancer of zeste homolog 2 Adenovirus (Ad-EZH2)
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.
BIOHIPPO AAV--CD68?150?-Cre-P2A-EGFP-WPREs
Recombinant adeno associated virus expressing Cre fused to EGFP under the CD68 promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
Vector Biolabs VEGF/VPF receptor Adenovirus (Ad-VEGF-R1)
Three cell membrane receptor tyrosine kinases Flt-1 (also designated VEGF-R1), Flk-1 (also designated VEGF-R2 or KDR) and Flt-4 (putatively involved in the growth of endothelial cells) are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domains. Two members of this receptor class, Flt-1 and Flk-1, represent high affinity receptors for vascular endothelial growth factor (VEGF). On the basis of structural similarity to Flt-1 and Flk-1, Flt-4 may represent a third receptor for either VEGF or a VEGF-related ligand. Flt-3/ Flk-2 and its human homolog, STK-1, are expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues.This is an adenovirus expressing human sFlt1.
Vector Biolabs Transmembrane 7 superfamily member 2 Adenovirus (Ad-SR-1)
Transmembrane 7 superfamily member 2 Adenovirus (Ad-SR-1)
Vector Biolabs Ca2/calmodulin-dependent protein kinase II gamma Adenovirus (Ad-CaMK2G)
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Six alternatively spliced variants that encode six different isoforms have been characterized to date. Additional alternative splice variants that encode different isoforms have been described, but their full-length nature has not been determined.
BIOHIPPO AAV-OT-ArchT-EGFP-WPREs
Recombinant adeno associated virus expressing ArchT fused to EGFP under the OT promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
IBA LifeSciences StrGate Acptr Vctr pCSG-IBA102
pCSG-IBA102 is a large expression vector with universal features for transient expression of target proteins with a C-terminal Twin-Strep-tag as well as for generation of stable mammalian cell lines. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells. The expressed protein is transferred into the medium due to the BM40 secretory signal peptide.
Vector Biolabs SMAD, mothers against DPP homolog 3 (Drosophila) Adenovirus (Ad-SMAD3)
Mammalian homologs of the Drosophila Mad gene include Smad1 (also designated Madr1 or JV4-1), Smad2 (also designated Madr2 or JV18-1), Smad3, Smad4 (also designated DPC4), Smad5, Smad6, Smad7 and Smad8 (also designated Smad9). Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGFb and activin-mediated growth modulation. Smad4 is required for all of the above activities and necessitates FAST-1 for DNA binding. Smad6 and Smad7 regulate the response to activin/ TGFb signaling by interfering with TGFb-mediated phosphorylation of other Smad family members.
Vector Biolabs Protein phosphatase 1A (formerly 2C), alpha isoform Adenovirus (Ad-PP1A)
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.
Vector Biolabs Platelet-derived growth factor, beta polypeptide Adenovirus (Ad-PDGF-B)
The term 'platelet-derived growth factor' (PDGF) refers to a family of disulphide-bonded dimeric isoforms that are important for growth, survival and function in several types of connective tissue cells. There are four members of the platelet-derived growth factor (PDGF) family; PDGF-A, PDGF-B, PDGF-C and PDGF-D. Their biological effects are mediated via two tyrosine kinase receptors, PDGFR-alpha and PDGFR-beta, and PDGF-mediated signaling is critical for development of many organ systems.
BIOHIPPO AAV-hSyn-DIO-NLS-Flp-P2A-EGFP-WPRE-hGH-polyA
Recombinant adeno associated virus expressing Flp fused to EGFP under the hSyn promoter with WPRE and hGH polyA elements. The Cre and Flp logic configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
SignaGen Laboratories AAV-GFP (AAV Serotype DJ/8), 1 x >30 uL
AAV(DJ/8)-CMV-GFP is a pre-packaged rAAV in serotype DJ/8 (with capsid from AAV serotype DJ/8 and 2xITR from AAV serotype 2) which over-expresses EGFP under CMV promoter. Used as a control and ready to use format.
New England Biolabs, Inc. M13mp18 RF I DNA – 10 µg
M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13. This phage vector contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the -Galactosidase gene. When a fragment of DNA is inserted into one of these sites, the -Galactosidase gene is inactivated, providing selection for clones on the appropriate indicator plate.