DNA Extraction and Purification
Bioworld Fungi/Yeast Genomic DNA Isolation Kit, 50 Prep(s)
For the rapid purification of DNA from yeast cells and fungal spores or mycelium No phenol required for isolation Purified DNA fully compatible with sensitive downstream applications Rapid and simple procedureFEATURES AND BENEFITSIsolate DNA from a wide range of samples -Genomic DNAcan be isolated from viable yeast cells, fungal spores or mycelium No phenol:chloroform extractions - DNAis isolated without the use of harmful chemicals such as phenol or chloroform Rapid and simple procedure - Rapid spin-column format allows for the processing of multiple samples in 45 minutes Isolate high yields of purified DNA - Recovered genomic DNA is suitable for downstream applications including PCR, Restriction Fragment Length Polymorphism (RFLP), and Amplified Fragment Length Polymorphism (AFLP)
Biochain Institute Inc FFPE Tissue DNA Extraction Kit — Magnetic Beads, 1 kit/pk
BioChain’s FFPE Tissue DNA Extraction Kit - Magnetic Beads allows for facile and efficient deoxyribonucleic acid extraction from FFPE tissues, with potential high throughput capabilities and full compatibility for down-stream applications such as qPCR. Utilizing heat and proteinase K treatment, BioChain’s FFPE Tissue DNA Extraction Kit- Magnetic beads is optimized in the removal of paraffin, artial reversal of formalin cross linking, and release of DNA from fixed tissues. After clean up with DNA inding beads, the concentration of the high urity DNA can be determined by spectrophotometer or anodrop equipment.
Enzo Life Sciences pJY2 plasmid (5µg)
The AGA arginine codon is rarely used in E. coli but is common in eukaryotic genes. The low level of tRNAUCUArg can lead to low expression and misincorporation of lysine for arginine, during expression of genes containing AGA codons in E. coli. E. coli harboring pJY2 can be employed to suppress lysine misincorporation and achieve high-level expression of genes cloned into pETvectors without modification of AGA codons in the target gene sequence. pJY2 encodes tRNAUCUArg (to suppress lysine misincorporation at AGA codons) and T7 lysozyme (to depress constitutive expression of the cloned gene). For example, pJY2 allows for faithful expression of pET3a-encoded mutant ubiquitins with a high density of AGA codons (up to 14% of total codons). pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase. Formulation: Liquid. In 10mM TRIS, pH 8.0, containing 1mM EDTA. Long Term Storage: -20°C.
Biochain Institute Inc Express Cloning Checker Kit II, 100 rxn/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Zymo Research Corporation DNA Clean & Concentrator™-5 (50 Preps) w/ Zymo-Spin™ I Columns (Uncapped)
The DNA Clean & Concentrator-5 (DCC-5) can also be used for post-RT cDNA clean-up and purification of sequencing-ready DNA from M13 phage. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ Column. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin column technology to yield DNA that is free of salts and contaminants in just 2 minutes. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures.
Biochain Institute Inc Genomic DNA - Rat Normal Tissue: Skeletal Muscle, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc FFPE and Frozen Matched Pair Genomic DNA: Human Tumor Tissue: Breast, 2 x 2 ug/pk
Matched Pair products include: Primary Pair (PP), or Primary and metastatic Pair (PM). PP consists of a pair of genomic DNA isolated from primary tumor and its adjacent normal tissue; PM consists of a pair of genomic DNA from primary tumor and corresponding metastatic tumor. Genomic DNA in each pair is prepared from the same donor. This product line is designed for identifying tumor-specific genes and tumor metastatic.