DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Asthma: Lung, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc SeqPure PCR Purification Kit (50 ml), 50 ml/pk
SeqPure PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
Biochain Institute Inc Genomic DNA - Human Adult Normal Tissue: Uterus, from a single donor, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Bioworld BAC DNA MiniPrep Kit, 25 Prep(s)
For the rapid preparation of BAC DNA High DNA recovery Alcohol-free protocol Convenient spin column protocol FEATURES AND BENEFITSHigh Recovery -0.6 to 1 µg of BAC DNA can be purified from 3.0 mL of bacterial culture. Alcohol-free protocol -No alcohols are used durig the purification process, thus the DNA is compatible with alcohol sensitive downstream applications. Fast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 1 hour. Recovered DNA is suitable for downstream applications - Purified BAC DNA is fully compatible with restriction enzyme digestions and sequencing. High binding capacity of columns -The binding capacity of the columns in the BAC DNA MiniPrep Kit is 25 µg
Qiagen, Inc. QIAAMP FAST DNA TISSUE KIT
NC3449504 QIAAMP FAST DNA TISSUE KIT
Zymo Research Corporation DNA/RNA Shield (25 ml) (2X Concentrate)
DNA/RNA Shield reagent preserves the genetic integrity and expression profiles of samples at ambient temperatures and completely inactivates infectious agents (viruses, bacteria, fungi, & parasites). The unique product also prevents degradation from freeze-thaw cycling and unexpected freezer failures.
Macherey-Nagel NEUTRALIZATION BUFFER NEU EF
Neutralization Buffer NEU EF to be used with NucleoBond Xtra Midi / Maxi EF kits
Zymo Research Corporation ZymoPURE Plasmid MiniPrep Kit (50 Preps)
The ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 g of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 g/l). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Zymo Research Corporation Quick-DNA™ Miniprep Plus Kit (200 Preps)
The Quick-DNA™ Miniprep Plus Kit is the easiest method for high yield total DNA extraction (e.g., genomic, plasmid, mitochondrial, viral) from any biological fluid,cell culture, or solid tissue sample. Innovative reagents and Zymo-Spin™ Technology allow for ultra-pure and concentrated genomic DNA 50 kb to be eluted in as little as 35 l. Zymo-Spin™ Columns ensure no buffer retention. Purified DNA is RNA-free, bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Macherey-Nagel Collection Tubes (1000)
Collection Tubes (1000) Collection Tubes 2 mL, without lid pack of 1000