DNA Extraction and Purification
Research Products International Corp ZR Plasmid Miniprep-Classic Kit (400 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
Research Products International Corp Quick-DNA Miniprep Plus Kit, 200 Preps
The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free,removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Quick & Easy: Simple 20 minute procedure.
Highest Yield: Recover 3x more DNA.
Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
Research Products International Corp Quick-DNA Plant/Seed Miniprep Kit, 50 Preps
The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology with Zymo-Spin IV-IR HRC filter. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
Ultra-high density BashingBeads are fracture resistant and chemically inert.
Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
Research Products International Corp Zymo ZymoPURE-Express Plasmid MidiPrep Kit, 25 Preps
The ZymoPURE - Express Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without the pelleting and resuspension steps. Just add the ZP Express Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the patented Zymo-Spin V-P microcentrifuge column. The ZymoPURE - Express Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. The innovative binding chemistry (patent pending) enables highly concentrated DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. The eluted plasmid DNA ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
Research Products International Corp DNA Clean & Concentrator-25, Capped, 200 Preps
The DNA Clean & Concentrator-25 (DCC-25) is a PCR purification kit designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin column. This PCR purification kit features Zymo-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc.
Column design allows DNA to be eluted at high concentrations into minimal volumes.
Research Products International Corp Zymoclean Gel DNA Recovery Kit w/ Zymo-Spin I Columns, Uncapped, 200 Preps
The Zymoclean Gel DNA Recovery Kit is a gel DNA extraction kit that provides rapid purification of high quality DNA from TAE/TBE-buffered agarose gels. This gel DNA extraction kit features Zymo-Spin technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean Gel DNA Recovery kits is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.
Quick recovery of ultra-pure DNA from agarose gels.
Column design permits DNA elution at high concentrations into minimal volumes.
Eluted DNA is well-suited for use in DNA ligation, sequencing, labeling, PCR, etc.
Research Products International Corp Quick-DNA MicroPrep w/Zymo-Spin IC Columns, Capped, 200 Preps
The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
Research Products International Corp Genomic DNA Clean & Concentrator-10 Kit, 25 Preps
The Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
Zymo Research Corporation Universal Methylated Human DNA Standard& Control Primers (Human)
The primer set is designed to amplify a fragment of the human MLH1 mismatch repair gene following bisulfite treatment. The methylated cytosines comprising CG dinucleotides remain unconverted following bisulfite treatment, whereas non-methylated cytosines are converted into uracil and detected as thymine after PCR.
Zymo Research Corporation Zyppy™ Plasmid Miniprep Kit (50 Preps)
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
Zymo Research Corporation EZ DNA Methlyation-Direct™ Kit (200 Rxns)
The EZ DNA Methylation-Direct kit is a bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Zymo Research Corporation ZymoBIOMICS™ MagBead (96 preps)
The provided DNA/RNA Shield™ inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method utilizes magnetic beads for DNA/RNA extraction without the use of phenol and is eluted into 50 l of ZymoBIOMICS™ DNase/RNase-Free Water. RNA is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
Beckman Coulter DNAdvance Kit - Genomic DNA extraction from tissue, 9,600 preps (A48706)
Beckman Coulter DNAdvance Kit (A48706) is a high-throughput genomic DNA extraction reagent kit that uses SPRI paramagnetic bead chemistry to isolate high-quality DNA from mammalian tissue samples. It is designed for automation on liquid-handling workstations and produces DNA compatible with PCR, qPCR, SNP genotyping, and next-generation sequencing.
- High-throughput capacity: 9,600 preps per kit.
- Technology: SPRI paramagnetic bead-based DNA isolation.
- Sample types: mammalian tissue including brain, kidney, liver, lung, and tail.
- Throughput: processes three 96-well plates in ~1 hour using automation; 15 minutes hands-on time for 96 samples; 1 hour 46 minutes total run time for a 96-well plate.
- Typical yield: isolates up to 40 μg DNA per prep.
- Compatible with downstream analyses: PCR, qPCR, SNP genotyping, sequencing.
Sigma Aldrich Fine Chemicals Biosciences Inactivated Escherichia coli Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | 1ML
Inactivated Escherichia coli Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml | 1ML
