DNA Extraction and Purification
Biochain Institute Inc CancerSeq Paraffin Tissue Tumor Block: Colon, 1 Block/pk
To generate our CancerSeq line of tissues, genomic DNA was extracted from the FFPE tissues and validated by targeted NGS with Illumina's TruSeq Amplicon - Cancer Panel (TSACP), which includes 48 cancer-related genes. Our prescreened tissues come with detailed information regarding donor and cancer type, as well as NGS information such as chromosomal position, variant type (SNP or indel), alternate allele variations, quality score, depth of coverage, allele type, transcript ID and more. We offer the prescreened tissue as mounted sections or whole block. Please inquire for other options, such as curls or extracted nucleic acids.
Biochain Institute Inc CancerSeq Paraffin Tissue Tumor Slides: Breast, 5 Slides/pk
To generate our CancerSeq line of tissues, genomic DNA was extracted from the FFPE tissues and validated by targeted NGS with Illumina's TruSeq Amplicon - Cancer Panel (TSACP), which includes 48 cancer-related genes. Our prescreened tissues come with detailed information regarding donor and cancer type, as well as NGS information such as chromosomal position, variant type (SNP or indel), alternate allele variations, quality score, depth of coverage, allele type, transcript ID and more. We offer the prescreened tissue as mounted sections or whole block. Please inquire for other options, such as curls or extracted nucleic acids.
Macherey-Nagel NucleoSpin cfDNA XS, 50 preps
NucleoSpin Plasma XS (50) 50 preps for the isolation of circulating DNA from plasma - NucleoSpin Plasma XS Columns, Collection Tubes, buffers, Proteinase K
Macherey-Nagel NucleoSpin DNA Lipid Tissue, Mini kit for DNA from lipid rich tissue, 50 preps/pk
Mini spin kit for isolation of DNA from lipid rich tissue, MN Bead Tubes Type D included.
Zymo Research Corporation Zymo Spin™ III-S Columns (25 Pack) w/ LuerLock Caps and 15 ml Reservoir
Compatible on a microcentrifuge or vacuum manifold for the purification of cell-free DNA. Features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 5 g of DNA in 35 l eluate. Capacity is 15 ml.
New England Biolabs, Inc. NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina - 96 rxn
The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of pre-fragmented DNA input amounts into libraries for next-generation sequencing on the Illumina platform all without an amplification step. The fast user-friendly workflow requires minimal hands-on time and maintains excellent library yields and GC coverage without requiring PCR. Dont be biased The NEBNext Ultra II DNA PCR-free Library Prep Kit for Illumina can save you time and minimize amplification-induced sequence bias and errors with a complete solution for PCR-free NGS library preparation. Built on the robust and reliable Ultra II reagents and workflow from New England Biolabs you can trust that your samples are in the best hands your own.
101 BIO Endotoxin-Free Plasmid Miniprep Kit
Eukaryotic cells are very sensitive to endotoxin, a common contaminant in the plasmid extraction, and the transfection efficiency of the cells will greatly reduce if the plasmid contains endotoxin. This kit can efficiently extract endotoxin-free plasmid in a fast and simple way. The endotoxin, genomic DNA, RNA, protein and other impurities can be largely removed. 3 ~30 μg transfection-grade plasmid DNA can be purified from 1 ~ 5 mL overnight culture of E. Coli in LB medium with this kit within 30 minutes. The extracted DNA can be directly used for Transfection PCR, restriction enzyme digestion, sequencing, etc. No need to precipitate, concentrate or desalt.
New England Biolabs, Inc. Monarch® HMW DNA Extraction Kits for Cells & Blood - 5 preps
The Monarch HMW DNA Extraction Kit for Cells & Blood provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from cultured cells and whole blood. Utilizing an optimized process that combines gentle cell lysis with a tunable fragment length generation followed by precipitation of the extracted DNA onto the surface of large glass beads, the prep proceeds rapidly and utilizes standard laboratory equipment. DNA size ranges from 50-250 kb for the standard protocol and into the Mb range when the lowest agitation speeds are used. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. For cells, the process time is only 30 min, while blood samples require erythrocyte lysis and are processed in 60 min
New England Biolabs, Inc. Monarch® HMW DNA Extraction Kit for Tissue - 50 preps
The Monarch HMW DNA Extraction Kit for Tissue provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from various tissues and bacteria, as well as other sample types including yeast, insect, and amphibian. Optimized extraction protocol for tissue utilizes pestle homogenization and proteinase K digestion with agitation for sample lysis, followed by a protein removal step and precipitation of the extracted DNA onto the surface of large glass beads. DNA size ranges from 50 to greater than or equal to 500 kb for standard protocols and into the Mb range when the lowest agitation speeds are used for processing soft organ tissues and bacteria. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. Purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing
New England Biolabs, Inc. Monarch® HMW DNA Extraction Kits for Cells & Blood - 50 preps
The Monarch HMW DNA Extraction Kit for Cells & Blood provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from cultured cells and whole blood. Utilizing an optimized process that combines gentle cell lysis with a tunable fragment length generation followed by precipitation of the extracted DNA onto the surface of large glass beads, the prep proceeds rapidly and utilizes standard laboratory equipment. DNA size ranges from 50-250 kb for the standard protocol and into the Mb range when the lowest agitation speeds are used. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. For cells, the process time is only 30 min, while blood samples require erythrocyte lysis and are processed in 60 min. Purified HMW DNA is suitable for a variety of downstream applications including long-read sequencing