DNA Extraction and Purification
Zymo Research Corporation EZ DNA Methlyation-Direct™ Kit (200 Rxns)
The EZ DNA Methylation-Direct kit is a bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
Molecular Research Center DNAzol, 200 mL
DNAzol is for use with tissues, cells and liquid samples. 1.0 ml of DNAzol isolates DNA from 25 - 50 mg tissue or from cell pellets containing 1 - 3 x 10^7 cells.
Research Products International Corp Quick-DNA Miniprep Plus Kit, 200 Preps
The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free,removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Quick & Easy: Simple 20 minute procedure.
Highest Yield: Recover 3x more DNA.
Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
Research Products International Corp Quick-DNA Plant/Seed Miniprep Kit, 50 Preps
The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology with Zymo-Spin IV-IR HRC filter. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
Ultra-high density BashingBeads are fracture resistant and chemically inert.
Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
Research Products International Corp Zymo ZymoPURE-Express Plasmid MidiPrep Kit, 25 Preps
The ZymoPURE - Express Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without the pelleting and resuspension steps. Just add the ZP Express Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the patented Zymo-Spin V-P microcentrifuge column. The ZymoPURE - Express Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. The innovative binding chemistry (patent pending) enables highly concentrated DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. The eluted plasmid DNA ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
Research Products International Corp Zymo Quick-DNA/RNA MagBead Kit, 96 Preps
The Quick-DNA/RNA MagBead Kit provides a high-throughput, magnetic-bead based purification of both high-quality DNA and total RNA (including small RNAs) from the same starting sample. The provided DNA/RNA Shield inactivates infectious agent and is ideal for sample storage at ambient temperatures. The extraction method has the option to recover total nucleic acids in one elution or DNA & RNA in separate elutions without the use of phenol. It is eluted into ≥50 µl of DNase/RNase-free water and is ready for any downstream application including RT-PCR, sequencing, etc.
Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
Research Products International Corp ZR Plasmid Miniprep-Classic Kit (400 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
Research Products International Corp DNA Clean & Concentrator-25, Capped, 200 Preps
The DNA Clean & Concentrator-25 (DCC-25) is a PCR purification kit designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin column. This PCR purification kit features Zymo-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc.
Column design allows DNA to be eluted at high concentrations into minimal volumes.
Research Products International Corp Quick-DNA MiniPrep w/Zymo-Spin IIC Columns, Capped, 50 Preps
The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells.
Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples.
Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
Research Products International Corp ZymoPURE Plasmid Miniprep Kit, 100 Preps
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
Research Products International Corp Zymoprep Yeast Plasmid Miniprep II w/Zymo-Spin I Columns, Uncapped, 50 Preps
The Zymoprep Yeast Plasmid Miniprep II is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, with no need for glass beads or phenol. The Zymoprep II recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.
Simple: Quickly and easily rescue plasmid from yeast.
Research Products International Corp DNA/RNA Shield, 2X Concentrate, 125 ml
DNA/RNA Shield reagent is a DNA and RNA stabilization solution for nucleic acids in any biological sample. This DNA and RNA stabilization solution preserves the genetic integrity and expression profiles of samples at ambient temperatures and completely inactivates infectious agents (viruses, bacteria, fungi, & parasites). The unique DNA and RNA stabilization solution also prevents degradation from freeze-thaw cycling and unexpected freezer failures.