DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Human Adult Normal Tissue: Peripheral Blood Leukocyte, from a single donor, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc FFPE Genomic DNA - Human Adult Normal Tissue: Liver, 10 ug/PK
>100K cancer & normal samples, lung, breast, colon, prostate, pancreas, melanoma BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Mouse Normal Tissue: Brain, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Control Genomic DNA - Human Male, 100 ug/PK
BioChain's control genomic DNAs are isolated from tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >;2.0. Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Control Genomic DNA - Cynomolgus Monkey Male, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Human Adult Normal Tissue: Liver, from a single donor, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Omega Biotek Inc E.Z.N.A. Plasmid Mini Kit I, Q(capless) Spin, 200preps
The E.Z.N.A.Plasmid Mini Kit I is designed to isolate up to 30 g of high quality plasmid DNA from 1 5 mL of bacterial cultures in 30 minutes or less. This kit uses a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Plasmid DNA purification is further simplified by using our HiBindmini column technology in three quick steps: bind, wash and elute. Purified plasmid DNA is ready for a wide variety of downstream applications including routine screening, restriction enzyme digestion, DNA sequencing, cloning, transformation and transfection. The E.Z.N.A.Plasmid DNA Mini Kit I is available with two different types of columns V-spin columns have an attached cap (D6943), while Q-spin columns are capless (D6942). The columns are otherwise identical in use and application. Either column can be used with both the vacuum or centrifugation protocol.
Omega Biotek Inc E.Z.N.A. FastFilter Plasmid Maxi Kit | 25 preps
The E.Z.N.A. FastFilter Plasmid Maxi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation.
Qiagen, Inc. QIAEX II GEL EXTRACT KIT (150)
Kit, Gel Extraction; Qiagen; QIAEX II (150); For batch purification of DNA fragments from agarose gels and solutions; Contains: 3 x 0.5mL QIAEX II Suspension, Buffers; For 150 extractions
Edge Biosystems Inc NEW PERFORMA DTR ULTRA 96-WELL
Microplate, 96-Well Kit; Edge Biosystems; Performa DTR Ultra; Dye terminator removal
Active Motif Nucleosome Preparation Kit
Active Motif's Nucleosome Preparation Kit is designed to prepare mono- and oligonucleosomes starting from cells. Nucleosomes comprise the smallest subunit of chromatin and consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Each nucleosome is separated by a linker of DNA. The Nucleosome Preparation Kit uses an enzymatic cocktail to digest the linker DNA. By adjusting the digestion time, it is possible to control the amount of mononucleosomes or oligonucleosomes extracted. Because isolated nucleosomes are more physiologically relevant than histones alone or synthetic peptides, they are ideally suited for use as substrates in the analysis of histone post-translational modifications, enzyme kinetics or inhibitor screening studies.
Macherey-Nagel NucleoBond PC 500, 25 preps
NucleoBond PC 500 (25) 25 preps for the isolation of plasmid DNA - NucleoBond AX 500 Columns, NucleoBond Folded Filters, buffers, RNase A
Norgen Biotek Corp Urine Cell-Free Circulating DNA Purification Maxi Kit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA as well as viral DNA from fresh, preserved or frozen urine samples from volumes ranging from 10 mL to 30 mL. The first column will handle the large volume input of urine that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. For a schematic workflow of the protocol click here.The purified urine DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, Microarrays and NGS.