RNA Preparation and Purification
Zyagen Labs Mouse C57 adrenal total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy C57BL/6 Mouse ADRENAL tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Testis, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Plant Normal Tissue: Rice, 100 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Plant Normal Tissue: Wheat, 100 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
New England Biolabs, Inc. NEBNext Low-biasSmallRNA-96rxn
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0 5 ng - 1 000 ng) or enriched small (0 05 ng - 5 ng) RNA The kit s reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample For optimal flexibility in multiplexing the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5) which must be purchased separately 480 indices are available
New England Biolabs, Inc. NEBNext Low-biasSmallRNA-24rxn
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0 5 ng - 1 000 ng) or enriched small (0 05 ng - 5 ng) RNA The kit s reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample For optimal flexibility in multiplexing the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5) which must be purchased separately 480 indices are available
Agilent Technologies ABSOLUTELY RNA NANOPREP KITAB
Absolutely RNA Nanoprep Kit 50 preps The Absolutely RNA nanoprep kit offers easy purification of DNA-free total RNA from the smallest samples eluted in a volume of 10microliter to provide RNA at a concentration useful for many downstream ap
Roche Diagnostics KAPA RNA HyperPrep Kit with RiboErase (HMR), 24 libraries
KAPA RNA HyperPrep Kit with RiboErase (HMR), 24 libraries. *This item is non returnable.
Norgen Biotek Corp RNA/Protein Purification Plus Kit, Spin column method, 30-35 μg RNA yield (10 mg tissue), 100-150 μg protein yield (10 mg tissue), 50 preps
The RNA/Protein Purification Plus Kit provides a rapid and efficient spin column-based method for the sequential isolation and purification of total RNA and proteins from biological samples without hazardous reagents. This kit enables researchers to conduct analyses of both the transcriptome and proteome from difficult-to-obtain samples, ensuring high-quality recovery of macromolecules.
- Rapid protocol with hands-on time under 30 minutes
- Efficient isolation of total RNA, including all sizes like mRNA and miRNA
- Compatible with various sample types: animal cells, tissues, blood, bacteria, yeast, fungi, and plants
- Includes dedicated gDNA removal technology for DNA-free RNA
- Yield of 30-35 μg RNA and 100-150 μg protein from 10 mg hamster liver
- Suitable for diverse downstream applications in RNA and protein analysis
- Shelf life of 1 year after shipment
- 50 preps included in the kit