RNA Preparation and Purification
Promega Biotinylated Oligo(dT) Probe (50pmol/μL)
The PolyATtract System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolation.
Promega SV 96 Total RNA Isolation System
The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells.
Promega PolyATtract™ System 1000 without Magnetic Stand
The PolyATtract System 1000 isolates messenger RNA directly from crude cell or tissue lysates, eliminating the need for total RNA isolation.
Promega Red Blood Cell Lysis Solution (CLB)
Component of the SV Total RNA Isolation and PureYield RNA Midiprep Systems.
Promega RNA Lysis Buffer
RNA Lysis Buffer (RLA) is used with several RNA purification systems to lyse cells.
Promega™ RNA Wash Solution (RWA)
RNA Wash Solution (RWA) is a component of the SV and SV 96 Total RNA Isolation System, PureYield RNA Midiprep System and ReliaPrep miRNA Cell and Tissue Miniprep System for preparation of RNA from tissues and cultured cells.
Promega PolyATtract™ System 1000 Magnetic Separation Stand
The PolyATtract(R) System 1000 Magnetic Separation Stand is designed for use with the PolyATtract(R) System 1000 and is ideal for applications requiring multiple paramagnetic isolations of biomolecules.
Promega PolyATtract™ mRNA Isolation System II with Magnetic Stand
The PolyATtract System isolates messenger RNA directly from total RNA.
Promega MagneSil™ Total RNA Mini-Isolation System
A high-throughput 96-well format for fast, easy preparation of intact total RNA from small amounts of cell culture, tissue culture cells, tissue or whole blood.
Beckman Coulter RNAClean XP, 40 mL
RNAClean XP 40 mL reagent kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions.
Zymo Research Corporation Direct-zol™ RNA MicroPrep (50 Preps) w/ Zymo-Spin™ IC Columns (Capped)
Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary.RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.Features:1. Efficient recovery of DNA-free Total RNAHigh-quality DNA-free RNA is purified from human epithelial cells using the Direct-zol™ procedure compared to a preparation from Supplier Q.2.Complete miRNA recoveryMicroRNA isolation can use Direct-zol™ RNA kits.The data show RNA purified from TRIzolsamples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion).MicroRNA analysis was performed using miRNAseq (MiSeq, Illumina) and direct hybridization assay (nCounter, Nanostring).
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (500 ug) - 10 preps
The Monarch Spin RNA Cleanup Kit (500 ug) reliably purifies up to 500 ug of concentrated, high-quality RNA (>25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our columns help ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nt). Monarch Spin RNA Cleanup Kits are also available for 10 ug and 50 ug binding capacities. Columns and buffers are also available separately for convenience
Takara Bio NucleoSpin RNA Blood Midi | 20 Preps
The NucleoSpin RNA Blood Midi kit enables efficient recovery of high-quality RNA from up to 1.3 ml of whole blood using a simple and convenient procedure that can be performed at room temperature. The kit protocol is compatible with common blood collection tubes and anticoagulants—including EDTA, citrate, and heparin—and enables processing of 6 samples in 75 minutes.