RNA Preparation and Purification
Molecular Research Center RNAzol RT, 500 mL
RNAzol RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.
System Biosciences LLC SeraMir Exosome RNA Purification Column Kit (20 exoRNA columns), 20 Preps
Efficiently purify RNA from already isolated exosomes with SBI’s most flexible SeraMir Kit.
New England Biolabs, Inc. Monarch Spin RNA Cleanup Kit (500 ug) - 10 preps
The Monarch Spin RNA Cleanup Kit (500 ug) reliably purifies up to 500 ug of concentrated, high-quality RNA (>25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our columns help ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nt). Monarch Spin RNA Cleanup Kits are also available for 10 ug and 50 ug binding capacities. Columns and buffers are also available separately for convenience
New England Biolabs, Inc. Monarch Spin RNA Cleanup Kit (50 ug) - 10 preps
The Monarch Spin RNA Cleanup Kit (50 ug) rapidly and reliably purifies up to 50 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 20 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in downstream applications including RT-PCR, RNA library prep for NGS and RNA labeling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch Spin RNA Cleanup kits are also available for 10 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience
Zymo Research Corporation Direct-zol™ RNA MicroPrep (50 Preps) w/ Zymo-Spin™ IC Columns (Capped)
Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary.RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.Features:1. Efficient recovery of DNA-free Total RNAHigh-quality DNA-free RNA is purified from human epithelial cells using the Direct-zol™ procedure compared to a preparation from Supplier Q.2.Complete miRNA recoveryMicroRNA isolation can use Direct-zol™ RNA kits.The data show RNA purified from TRIzolsamples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion).MicroRNA analysis was performed using miRNAseq (MiSeq, Illumina) and direct hybridization assay (nCounter, Nanostring).
Norgen Biotek Corp Plant/Fungi Tot Rna Our 50 Pre
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient. The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
Molecular Research Center RNAZOL RT REAGENT 200ML
A single-step reagent isolating mRNA and small RNA in separate fractions. No chloroform-induced phase separation or refrigerated centrifuge required. No DNase treatment. Higher yields and quality than previous single-step methods.
Zymo Research Corporation Direct-zol™ RNA MicroPrep (200 Preps) w/ Zymo-Spin™ IC Columns (Capped)
Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary.RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.Features:1. Efficient recovery of DNA-free Total RNAHigh-quality DNA-free RNA is purified from human epithelial cells using the Direct-zol™ procedure compared to a preparation from Supplier Q.2.Complete miRNA recoveryMicroRNA isolation can use Direct-zol™ RNA kits.The data show RNA purified from TRIzolsamples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion).MicroRNA analysis was performed using miRNAseq (MiSeq, Illumina) and direct hybridization assay (nCounter, Nanostring).
BIOSEARCH TECHNOLOGIES INC (Lucigen) NxGen RNAse Inhibitor, 10,000 U @ 40 U/LEliminate RNase activity.
RNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.Supplied at 40,000 U/mL in 20 mM HEPES-KOH, 50 mM KCl, 8 mM dithiothreitol, 50% glycerol, pH 7.5 @ 25¿C.
Apexbio Technology LLC MRNA SYN ARCA T7 25RXN
HyperScribe All in One mRNA Synthesis Kit (ARCA T7 poly(A)) is designed for rapid in vitro generation of capped and polyadenylated mRNA transcripts Utilizing T7 RNA polymerase this system incorporates Anti-Reverse Cap Analog (ARCA) a Cap0 analog co-transcriptionally at the 5 terminus to confer mRNA stability and enhance translational efficiency After template DNA removal via DNase I treatment Poly(A) polymerase adds a poly(A) tail at the 3 -end further stabilizing the transcript and increasing translation initiation efficiency This kit is employed in research involving translation systems antisense RNA RNA interference RNA vaccines structural RNA studies and ribonuclease assays
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 1 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.