RNA Preparation and Purification
Molecular Research Center TRI REAGENT 500 ML
Reagent, Tri; Molecular Reaearch Centre; For RNA isolation; Isolation time: Less than one hour; 50mm sufficient to process 50 samples; 50 to 100mg of tissue each; 500mL
Zymo Research Corporation Quick-RNA MagBead (96 preps)
Total RNA is eluted into 50 l of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT/PCR, hybridization, etc.
Norgen Biotek Corp RNase-Free DNase I Kit, 50 Reactions
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Norgen Biotek Corp Animal Tissue RNA Purification Kit
This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, and intestine. The tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, NGS and more.The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Sigma Aldrich Fine Chemicals Biosciences RNAlater Stabilize and protect RNA with immediate RNase inactivation | MFCD03453003 | 500ml
RNAlater Stabilize and protect RNA with immediate RNase inactivation | MFCD03453003 | 500ml
![Zymo Research Corporation Direct-zol™ RNA MicroPrep (50 Preps) w/ Zymo-Spin™ IC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 x 1 - packaged separately]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-R2061DIRECTZOLRNAMICROPREPYES.JPG-250.jpg)
Zymo Research Corporation Direct-zol™ RNA MicroPrep (50 Preps) w/ Zymo-Spin™ IC Columns (Capped) (Product Supplied w/ 50 ml TRI Reagent™) [Includes R2050 x 1, R2050-1-50 x 1 - packaged separately]
Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary.RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.Features:1. Efficient recovery of DNA-free Total RNAHigh-quality DNA-free RNA is purified from human epithelial cells using the Direct-zol™ procedure compared to a preparation from Supplier Q.2.Complete miRNA recoveryMicroRNA isolation can use Direct-zol™ RNA kits.The data show RNA purified from TRIzolsamples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion).MicroRNA analysis was performed using miRNAseq (MiSeq, Illumina) and direct hybridization assay (nCounter, Nanostring).
Molecular Research Center RNAzol RT, 500 mL
RNAzol RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.
Norgen Biotek Corp Plant/Fungi Tot Rna Our 50 Pre
Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient. The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Norgen's Plant/Fungi Total RNA Purification Kit is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (10 ug) - 10 preps
The Monarch Spin RNA Cleanup Kit (10 ug) rapidly and reliably purifies up to 10 ug of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions including labeling, capping, in vitro transcription (IVT) and DNase I treatment. The kit can also be applied for total RNA extraction from some sample types. This kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design helps ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 ul. Eluted RNA is ready for use in a variety of downstream applications, including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nts). Monarch Spin RNA Cleanup kits are also available for 50 ug and 500 ug binding capacities. Columns and buffers are also available separately for convenience
Macherey-Nagel NUCLEOSPIN RNA CLEAN UP MXI KT
NucleoSpin RNA Clean up Maxi kit for RNA clean up and concentration
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Lung: Right Lower Lobe, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.