Miscellaneous Molecular Biology Reagents
Sigma Aldrich Fine Chemicals Biosciences MES Monohydrate | 145224-94-8 | MFCD00149409 | 10g
MES Monohydrate | Purity: 99% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 10g
Sigma Aldrich Fine Chemicals Biosciences PHENOL:CHLOROFORM:ISOAMYL100ML
Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA 100mL
MOLTOX Molecular Toxicology Inc Tryptic Soy Broth (TSB) w/0.5% Lecithin & 4% Tween 80, pH 7.2 +/-0.2, 90 mL/bottle, Store in refrigerator
This item has a minimum order quantity of 96 per supplier requirements. Also known as Trypticase or Soybean-Casein Digest, Tryptic Soy Broth is a general purpose medium for the cultivation of fastidious and nonfastidious microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi. Whether you need a general-purpose, nonselective media or you need added blood to facilitate a more fasitdious bacterial lawn for the detection of hemolytic reactions, we have several products to choose from. MOLTOX does not provide microorganisms for testing. Some media are temperature sensitive and may have specific temperature and storage requirements for shipping/delivery.
Hampton Research HR3-133/I3C Phasing Kit - 12 aliquots of I3C (280 mg each) and 12 aliquots of 2.0 M Lithium hydroxide (650 ul each)
HR3-133/I3C Phasing Kit - 12 aliquots of I3C (280 mg each) and 12 aliquots of 2.0 M Lithium hydroxide (650 ul each). Please note items from this supplier are non-returnable.
New England Biolabs, Inc. SNAP-Cell Block – 100 nmol
SNAP-Cell Block (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-tag in solution or in living cells.
New England Biolabs, Inc. SNAP-Capture Magnetic Beads – 2 ml
The SNAP-Capture Magnetic Beads are used to selectively immobilize and magnetically separate a SNAP-tag fusion protein from solution using magnetic agarose beads. They are prepared by the coupling of SNAP-tag substrate benzylguanine with highly stable 20-100 um superparamagnetic particles.
New England Biolabs, Inc. EcoRV-HF – 4000 units
A high fidelity restriction endonuclease that recognizes the sequence GAT_^ATC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. SspI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence AAT_^ATT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Endonuclease IV – 5000 units
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5' to the lesion leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5' terminus. The enzyme also has a 3' -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5'-phosphate and phosphate from the 3' end of DNA.
New England Biolabs, Inc. SNAP-Cell 505-Star – 50 nmol
SNAP-Cell 505-Star is a photostable green fluorescent substrate that can be used to label SNAP-tag fusion proteins inside living cells, on cell surfaces, or in vitro.
![Research Products International Corp EGTA [Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-E57060-50.0.JPG-250.jpg)
Research Products International Corp EGTA [Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid], 50 Grams
- CAS Number: 67-42-5
- Molecular Formula: C14H24N2O10
- Molecular Weight: 380.35
Enzo Life Sciences FLUOR DE LYS developer I concentrate (300 µl)
An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS substrate. FLUOR DE LYS Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS substrate. An appropriate FLUOR DE LYS family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins). Shipping: Shipped on Blue Ice. Long Term Storage: -80°C