Miscellaneous Molecular Biology Reagents
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Endothelial Cells
In addition to various cell type-specific kits for primary endothelial cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian endothelial cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for endothelial cells. Benefits:Optimization can be performed within 1 experiment Detailed protocol guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support Team Transfection efficiencies up to 90%Viabilities up to 85%Applications:Kit suited for endothelial cells from different mammalian species and various organsAlready tested for human pulmonary artery endothelial cells
Sigma Aldrich Fine Chemicals Biosciences BM-Purple
BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate SOLUTIONon may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.Appearance prior to use light yellow color After color development dark purple
MOLTOX Molecular Toxicology Inc NADPH REGENSYS A, 50 mL size refers to final volume of S9 mix per pkg, G-6-P MgCl2-KCL in 0.1M Phosphate Buffer, pH 7.4, Store in Refrigerator
Convenient preformulated filter sterilized and prepackaged S9 mix components for preparation of 4 - 10% S9 mix. Regensys™ """"A"""" is formulated as described by Maron & Ames, Mut. Res. 113: 173, 1983. Size refers to final volume of S9 mix. Regensys™ """"B"""" consists of accurately weighed aliquots of crystalline NADP to add to corresponding Regensys™ """"A"""" for completion of the Regensys™ system. MOLTOX S9 preparations and cofactor reagents for use in metabolic activation studies are manufactured to rigorous standards of quality and performance and are used by leading industrial, government and academic laboratories worldwide.
Hampton Research HR2-993-28/StockOptions Bis-Tris Propane #28 pH 9.0, 185 ml
HR2-993-28/StockOptions Bis-Tris Propane #28 pH 9.0, 185 ml. Please note items from this supplier are non-returnable.
Sigma Aldrich Fine Chemicals Biosciences 3, 3',5 ,5'-TETRAMETHYLBENZIDI
3,3',5 ,5'-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA
Bioworld DMSO Bio-Max, Molecular Biology Grade, 67-68-5, 100ml
DMSO (Dimethyl sulfoxide) is a polar solvent used in many chemical reactions, solubilization of peptides, in polymerase chain reactions (PCR) and as a cryoprotectant agent for the preservation of cells. Biomax DMSO is used in cell freezing media to protect cells from ice crystal formation causing mechanical injury to the cells. DMSO is often used in the combinations with BSA or fetal bovine serum (FBS) in various media. Technical Specifications: Tissue Culture Grade, DMSO, Sterile filtered, Cell culture tested. For use in cell freezing. Appearance: Liquid, Boiling Point : 189 C(lit.), Density : 1.10 g/mL(lit.), Melting Point: 16-19 C(lit.), HS Code: 2930.90.70, Molecular Weight : 78.13, Molecular Formula : C2H6OS, Purity : >99.5%, Solubility : Soluble in water, ethanol, ether, chloroform, benzene, and acetone. Appearance Form : Liquid. Appearance Color : Colorless to faint yellow. Melting Point : 16 - 19C, Boiling Point : 189C, Density : 1.10 g/mL, 100ml
Genscript Corporation SurePAGE™, Bis-Tris, 10x8, 10%, 10 wells
GenScript SurePAGE, Bis-Tris, 10 cm x 8 cm gels are high-performance precast mini polyacrylamide gels with a special design that allows large sample loading volumes. The unique formulation of the gel and cassette design enables superior band resolution and significantly improved band evenness. SurePAGE gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis, increases gel stability and minimizes protein modification. SurePAGE gels guarantee excellent batch-to-batch consistency and a reliable protein migration pattern. With specially formulated Tris-MOPS running buffer, proteins can be separated quickly and efficiently for subsequent detection by staining or Western blotting. SurePAGE, Bis-Tris, 10x8 gels are available in gradient (4-20%, 4-12%, and 8-16%) and homogeneous (8%, 10%, 12% and 15%) concentrations. Each gel concentration has comb configurations of 10-well, 12-well and 15-well.
Sigma Aldrich Fine Chemicals Biosciences Duolink(R) In Situ Probemaker PLUS | 1KT
Duolink(R) In Situ Probemaker PLUS | 1KT
Supply Solutions Sigma Aldrich - Guanidine Hydrochloride; Size: 100g; CAS Number: 50-01-1
Sigma Aldrich - Guanidine Hydrochloride; Size: 100g; CAS Number: 50-01-1
Quality Biological Inc Water, Molecular Biology, PK 4 X 100 mL
QBI's Molecular Biology Water is prepared by reverse osmosis ¿ Passed through fine carbon ¿ Deionized through two resin beds ¿ Serially filtered through 0.1 ¿m positively charged membranes ¿ Endotoxin level less than 0.03 EU/mL. ¿ Applications: Useful in reconstituting nucleic acids and/or proteins, RNA applications (i.e. Preparation of an RNA probe), DNA applications (i.e. Subcloning in pUC plasmids), and protein applications (i.e. Western Blotting). ¿ Also, useful as a diluent or solvent. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered & Deionized ¿ Endotoxin (<0.03 EU/mL) and Mycoplasma Tested ¿ DNase, RNase, and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 41116134 ¿ ¿ Max Shelf Life: 36 months
ProteinSimple Maurice Sample Vials with Integrated inserts, 0.2 mL, 100/pk
Vials with integrated inserts for samples, accomodates 200 uL sample volume. For use with Maurice, Maurice S., and Maurice C. systems only.
Sigma Aldrich Fine Chemicals Biosciences Pefabloc SC, 100 mg
Synonym: 4-(2-aminoethyl)-benzene-sulfonyl fluoride, aebsf, aminoethyl-benzene-sulfonyl fluoride, 4-2-, proteinase k inhibitor. Pefabloc SC is used to inhibit the detrimental effects of proteases during preparative protein purification. Due to its low toxicity toward eukaryotic cells, it may be applied in the production of recombinant proteins, during fermentation of transformed cells, where proteolytic digestion may decrease the yield of the desired product.