Miscellaneous Molecular Biology Reagents
New England Biolabs, Inc. SspI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence AAT_^ATT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Endonuclease IV – 5000 units
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5' to the lesion leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5' terminus. The enzyme also has a 3' -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5'-phosphate and phosphate from the 3' end of DNA.
Apexbio Technology LLC 12-O-tetradecanoyl phorbol-13-acetate (PMA),5mgCAS# 16561-29-8
12-O-tetradecanoyl phorbol-13-acetate (TPA) is an activator of ERK/MAPK with the concentration of 50μm [1].ERK is an extracellular signal-regulated kinase and transfers signals from a receptor on the cell surface to DNA cooperated with MAPK. It is reported that ERK deficiency leads to the cell uncontrolled growth and is regarded as a target to cure cancers.TPA is a potent ERK activator. When tested with A549 cells (human lung cancer cell line), TPA treatment led to an early, strong, and relatively transient ERK phosphorylation [2]. In mouse embryo fibroblasts from DUSP5 (+/+) mice, administration of TPA increased levels of ERK expression [3]. Other sizes are also available. Please inqury us for quote.
New England Biolabs, Inc. SNAP-Capture Magnetic Beads – 2 ml
The SNAP-Capture Magnetic Beads are used to selectively immobilize and magnetically separate a SNAP-tag fusion protein from solution using magnetic agarose beads. They are prepared by the coupling of SNAP-tag substrate benzylguanine with highly stable 20-100 um superparamagnetic particles.
New England Biolabs, Inc. EcoRV-HF – 4000 units
A high fidelity restriction endonuclease that recognizes the sequence GAT_^ATC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. SNAP-Cell 505-Star – 50 nmol
SNAP-Cell 505-Star is a photostable green fluorescent substrate that can be used to label SNAP-tag fusion proteins inside living cells, on cell surfaces, or in vitro.
STEMCELL Technologies ALDEFLUOR™ DEAB Reagent, 1.5 mM, 1 mL, Size: 1 mL
Use ALDEFLUOR™ DEAB Reagent as a negative control to account for background fluorescence in the ALDEFLUOR™ assay system.Available either individually or as part of the ALDEFLUOR™ Kit (/products/aldefluor-kit.html), this diethylaminobenzaldehyde (DEAB) reagent is an aldehyde dehydrogenase (ALDH) enzyme inhibitor and can be used during the identification of ALDH-bright (ALDHbr) cells.View our additional resources to learn more about the ALDEFLUOR™ reagent system (/products/brands/aldefluor.html).
ENCAPSULA NANAOSCIENCE LLC Encapsome 10mL
Control liposomes (Encapsome) are recognized and phagocytosed by the same mechanism as Clodrosome. Since the control liposomes do not contain clodronate, the phagocytic cells are not killed. However, phagocytes do respond to the ingestion of control liposomes by cytokine secretion, temporary suspension of phagocytic activity.
Cayman Chemical PulsatIla SaponIn D 5mg
An oleanane-type saponin with anticancer activity; cytotoxic to A549, SKOV3, SK-MEL-2, and HCT15 cells (EC50s = 11.25, 13.17, 3.04, and 11.86 μM, respectively); reduces tumor volume in a murine Lewis lung carcinoma model at 6 mg/kg per day
Crescent Chemical Co Inc SDS SOLUTION 20%
SDS Solution, 20 % SDS (cat. no. 20770): 200 g/L Size - 1L Storage Conditions - +15 °C TO +30 °C Catalog Number - 20767.03
Alkali Scientific CellPro™ EDTA, 0.5M, pH 8.0, 100mL
Alkali Scientific™ presents CellPro™ EDTA, Molecular Biology Grade, a high-quality solution designed for molecular biology applications. This molecular biology-grade EDTA offers exceptional purity and reliability, ensuring optimal performance in a variety of laboratory procedures. With its sterile formulation and molecular biology-grade quality, CellPro™ EDTA guarantees the preservation of sample integrity and the prevention of nucleic acid degradation. Trust CellPro™ EDTA for consistent and reproducible results in your molecular biology experiments.
Alkali Scientific CellPro™ EGTA. 0.5M, pH8.0, 100mL
Alkali Scientific™ CellPro™ EGTA, Molecular Biology Grade, is a meticulously purified compound designed to meet the stringent requirements of molecular biology and biochemistry applications. Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetra acetic acid (EGTA) is a chelating agent widely used for its ability to bind divalent cations, particularly calcium ions, effectively. This high-quality EGTA is meticulously prepared to ensure minimal impurities, making it suitable for critical molecular biology experiments such as nucleic acid and protein studies. With its exceptional purity and consistency, CellPro™ EGTA guarantees reliable and reproducible results in a wide range of research applications. Trust in the quality and performance of CellPro™ EGTA to support your molecular biology endeavors with precision and confidence.
Hampton Research HR2-993-01/StockOptions Bis-Tris Propane #01 pH 6.3, 185 ml
HR2-993-01/StockOptions Bis-Tris Propane #01 pH 6.3, 185 ml. Please note items from this supplier are non-returnable.
Alkali Scientific CellPro™ EGTA 0.5M pH 8.0, 50mL
Alkali Scientific™ CellPro™ EGTA, Molecular Biology Grade, is a meticulously purified compound designed to meet the stringent requirements of molecular biology and biochemistry applications. Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetra acetic acid (EGTA) is a chelating agent widely used for its ability to bind divalent cations, particularly calcium ions, effectively. This high-quality EGTA is meticulously prepared to ensure minimal impurities, making it suitable for critical molecular biology experiments such as nucleic acid and protein studies. With its exceptional purity and consistency, CellPro™ EGTA guarantees reliable and reproducible results in a wide range of research applications. Trust in the quality and performance of CellPro™ EGTA to support your molecular biology endeavors with precision and confidence.