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Nucleotides are composed of a nitrogenous base covalently bound to a 5-carbon sugar molecule and one or more phosphate functional groups. They are the molecules that make up the structural units of DNA and RNA.
CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs). These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. They have been shown to induce a coordinated set of immune responses based on the activation of immune cells primarily involved in the recognition of these molecules.
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RNA methylation signals a plethora of cellular functions including biochemical and metabolic stabilization of RNA, immune response, resistance to antibiotics, mRNA reading frame maintenance, splicing and more.
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5-hme-CTP (5-hydroxymethylcytidine triphosphate) is a modified cytidine triphosphate bearing a hydroxymethyl group at the 5-position carbon This modification can enhance RNA stability and functionality During in vitro mRNA synthesis 5-hme-CTP can be incorporated into RNA molecules producing RNA containing 5-hydroxymethylcytidine Hydroxymethyl-modified RNA plays an essential role in studying RNA structure function and RNA-protein interactions Additionally 5-hme-CTP significantly improves RNA stability by reducing its intracellular degradation Thus this modified nucleotide has broad applications in gene expression research RNA-based drug development and gene therapy
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Aminoallyl-dUTP-X-Cy5 is a fluorescently labeled deoxynucleotide analogue structurally resembling natural thymidine triphosphate (dTTP) It serves as a substrate suitable for enzymatic incorporation into DNA strands through methods such as PCR reverse transcription nick translation or random priming Upon incorporation this molecule confers red fluorescence (excitation/emission 650/665 nm) to nucleic acid probes facilitating their detection in applications including in situ hybridization microarray assays blotting procedures and multiplex fluorescence analyses
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CpG ODN 10101 is a solid laboratory chemical with a molecular weight of 6702.37. Identified by CAS No. 1234402-77-7, this substance is stable under recommended storage conditions. It is intended for research use only and should be handled by suitably qualified and experienced scientists in appropriately equipped facilities.
Solid appearance
Molecular weight of 6702.37
Stable under recommended storage conditions
Suitable for laboratory chemical applications
Not classified as a hazardous substance or mixture
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5-FAM-Alkyne is an alkyne-functionalized fluorescein derivative supplied as a solid fluorescent labeling reagent for copper-catalyzed click chemistry. It is designed for conjugation to biomolecules and use in fluorescence-based detection and imaging applications, providing reliable labeling in typical biochemical workflows.
Alkyne functional group for click chemistry.
High purity (95.0%).
Solid form, yellow to brown.
Molecular weight 413.38 g/mol.
Suitable for fluorescent labeling and imaging applications.
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2-Thio-UTP is a base-modified ribonucleoside triphosphate commonly used in RNA-based biomedical research Incorporation of 2-Thio-UTP into RNA transcripts reduces innate immune activation compared to unmodified RNA Mechanistically RNAs transcribed with 2-Thio-UTP lack the ability to activate RNA-dependent protein kinase (PKR) markedly diminish 2 -5 -oligoadenylate synthetase (OAS) pathway induction decrease recognition by retinoic acid-inducible gene I (RIG-I) and suppress Toll-like receptor (TLR) mediated signaling Thus 2-Thio-UTP serves as a research tool in studies designing low-immunogenic RNAs for therapeutic applications
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