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Sequenced positive and negative RNA controls and RNA control mixes collected from various organisms. Products include mRNA, sgRNA, tracrRNA, and crRNA and are ideal for use in gene expression experiments.
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ER-EGFP mRNA encodes a fusion protein comprising an endoplasmic reticulum (ER) targeting signal sequence and enhanced green fluorescent protein (EGFP) EGFP is a widely used fluorescent marker protein for biological imaging By fusing EGFP to the ER signal sequence dynamic changes in the ER can be observed in real-time in live cells making it particularly useful for studying the structure function and interactions of the ER with other cellular organelles ER-EGFP s efficient fluorescent properties and specific localization to the ER position it as an ideal tool for studying ER-related processes The EZ Cap ER-EGFP Probe mRNA (m1 ) is provided at 1 mg/mL specifically designed for targeted expression of a green fluorescent protein label at the ER This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo
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Firefly Luciferase mRNA (ARCA 5mCTP UTP) encodes firefly luciferase protein originally isolated from Photinus pyralis Firefly luciferase is a widely used bioluminescence reporter molecule for studies on gene regulation cell viability ATP levels and in vivo imaging In the presence of ATP and oxygen luciferase catalyzes the oxidation of D-Luciferin (Cat C3654) emitting chemiluminescence at 560 nm This product is a 5 -capped 3 -poly(A)-tailed mRNA modified with 5mCTP and UTP mimicking mature eukaryotic mRNA Capped with ARCA (anti-reverse cap analog Cat B8175) via co-transcription it forms a Cap 0 structure enhancing stability and translation efficiency [1 2] The 5mCTP (Cat B7967) and UTP (Pseudo-UTP Cat B7972) modifications reduce innate immune activation and increase mRNA stability The poly(A) tail boosts stability and translation initiation efficiency
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The luciferase-luciferin bioluminescent system provides a highly sensitive and efficient tool for detecting gene expression In the presence of ATP oxygen and Mg2 firefly luciferase catalyzes the oxidation reaction of luciferin into oxyluciferin accompanied by the emission of bioluminescence at about 560 nm following detection by a luminometer or liquid scintillation counter The Single Firefly Luciferase Reporter Gene Assay Kit allows direct quantification of intracellular firefly luciferase activity via chemiluminescence without the need for washing or harvesting cells This assay is a one-step detection kit providing exceptional sensitivity high signal stability and ease of use This product is an alternative lyophilized powder version of Kit (K2236) with the same performance offering the benefit of long-term stable storage at -20 C Before use the lyophilized substrate powder needs to be fully dissolved with the buffer provided in the kit to prepare working solution
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More