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Short/small hairpin RNA (shRNA) is an artificial RNA molecule with a tight hairpin turn used to silence target gene expression via RNA interference (RNAi). Cells can be made to express shRNA through the introduction of plasmids or viral/bacterial vectors.
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The OriGene Technologies TR30021V5 is a lentiviral control particle that functions as a negative control in shRNA experiments, featuring a GFP label for easy visualization. Engineered with a puromycin resistance gene, it allows efficient selection of transduced mammalian cells. This product aids in optimizing multiplicity of infection (MOI), providing researchers with essential tools for successful gene delivery.
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This gene encodes a receptor for prostaglandin E2, a metabolite of arachidonic acid which has different biologic activities in a wide range of tissues. Mutations in this gene are associated with aspirin-induced susceptibility to asthma.
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Lentiviral Packaging Mix is a ready-to-use third generation HIV-based lentiviral packaging system in which the plasmids express the elements required for lentiviral production. To produce high quality lentiviral particles, all you need is a lentiviral expression vector containing your gene of interest. Lentiviral Packaging Mix contains the essential packaging vectors containing the viral structure proteins and the envelope vector to express the Vesicular Stomatitis Virus glycoprotein (VSVG). The lentiviral packaging mix is TAT-independent because it only supports lentiviral expression vector with a chimeric 5' LTR, in which the HIV promoter is replaced with CMV or RSV.
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The protein encoded by this gene is a serine threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome as well as with skin pancreatic and testicular cancers
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The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation Ubiquitination involves at least three classes of enzymes ubiquitin-activating enzymes ubiquitin-conjugating enzymes and ubiquitin-protein ligases This gene encodes a member of the E2 ubiquitin-conjugating enzyme family The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression and may be involved in cancer progression Multiple transcript variants encoding different isoforms have been found for this gene Pseudogenes of this gene have been defined on chromosomes 4 14 15 18 and 19
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