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Short/small hairpin RNA (shRNA) is an artificial RNA molecule with a tight hairpin turn used to silence target gene expression via RNA interference (RNAi). Cells can be made to express shRNA through the introduction of plasmids or viral/bacterial vectors.
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Lentiviral Packaging Mix is a ready-to-use third generation HIV-based lentiviral packaging system in which the plasmids express the elements required for lentiviral production. To produce high quality lentiviral particles, all you need is a lentiviral expression vector containing your gene of interest. Lentiviral Packaging Mix contains the essential packaging vectors containing the viral structure proteins and the envelope vector to express the Vesicular Stomatitis Virus glycoprotein (VSVG). The lentiviral packaging mix is TAT-independent because it only supports lentiviral expression vector with a chimeric 5' LTR, in which the HIV promoter is replaced with CMV or RSV.
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The protein encoded by this gene is a member of the dual specificity protein kinase family which acts as a mitogen-activated protein (MAP) kinase kinase MAP kinases also known as extracellular signal-regulated kinases (ERKs) act as an integration point for multiple biochemical signals This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals As an essential component of MAP kinase signal transduction pathway this kinase is involved in many cellular processes such as proliferation differentiation transcription regulation and development
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The OriGene Technologies Human shRNA Lentiviral Plasmid Kit efficiently targets Myostatin gene expression in human cells, enabling both transient and stable gene knockdown. With a guarantee of ≥70% knockdown efficiency, this kit includes sequence-verified shRNA constructs and a third-generation lentiviral vector, featuring a selection marker and fluorescent reporter for easy identification of transduced cells. It's suitable for researchers looking to effectively manipulate gene expression.
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