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Small interfering RNA/silencing RNA (siRNA) is a class of double-stranded, non-coding RNA molecule, 20-25 base pairs in length. As part of the RNA interference pathway, it regulates gene expression by degrading mRNA after transcription, preventing translation.
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008].
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A novel siRNA for difficult-to-transfect cells, modified to require no transfection reagent or viral vector for delivery. Predesigned Accell siRNA is available as individual reagents and in SMARTpool format.
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Accell siRNA offers what no other RNAi product can claim: delivery into difficult-to-transfect cells without transfection reagents, virus, or instrumentation. Achieve targeted gene silencing in neuronal, immune, or primary cells that are beyond the reach of conventional RNAi products due to toxicity caused by transfection reagents or undesirable viral responses.
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Target species: human; for corresponding mouse product, see GPR75 siRNA and shRNA Plasmids (m)Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in lengthFor independent verification of GPR75 gene silencing results, individual duplex components or plasmids are also available upon requestGet details about si/shRNA Gene SilencersGene silencers are intended for noncommercial and research use only.
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