Crosslinking Reagents
Thermo Scientific™ Sulfo-EMCS (N-ε-maleimidocaproyl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of a water-soluble, 8-atom (9.4A) spacer arm.
Thermo Scientific™ EZ-Link™ Biotin-PEG12-DBCO
EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach.
Thermo Scientific™ BS2G-d0 (bis(sulfosuccinimidyl) glutarate-d0)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of a 7.7A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientific™ L-Photo-Methionine
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
Thermo Scientific™ L-Photo-Leucine
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.
Thermo Scientific™ NHS-Azide
Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.
Thermo Scientific™ NHS-Phosphine
Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.
Thermo Scientific™ EZ-Link™ TFP Ester-PEG12-DBCO
A heterobifunctional crosslinker that contains an amine reactive tetrafluorophenyl (TFP) and a dibenzylcyclooctyne (DBCO) with a water solubilizing long PEG12 spacer in between. Use to label any primary amine containing molecule such as a protein to biomolecule with a distantly attached DBCO group.
Thermo Scientific™ DTME (dithiobismaleimidoethane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.
Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))
Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.
![Thermo Scientific™ SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)](https://assets.fishersci.com/TFS-Assets/LSG/product-images/23013-SPB.jpg-250.jpg)
Thermo Scientific™ SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)
Crosslink amines to DNA with this crosslinker composed of NHS-ester and psoralen reactive groups; conjugates to DNA via light-activated intercalation next to pyrimidine bases.
Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
Optimize applications where product integrity and risk minimization are paramount our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.
Thermo Scientific™ SMPH (Succinimidyl 6-((beta-maleimidopropionamido)hexanoate))
Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 12-atom (14.2A) spacer arm.
Thermo Scientific™ PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)
Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ BMPH (N-β-maleimidopropionic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this short (8.1A) crosslinker containing maleimide and hydrazide groups.