Crosslinking Reagents
Thermo Scientific™ Mass Spectrometry Cleavable Crosslinkers
Improve sensitivity of mass spectrometry detection of crosslinked proteins with MS-cleavable DSSO and DSBU heterotrifunctional crosslinkers.
Thermo Scientific™ Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) (sulfosuccinimidyl 6-(4,4'-azipentanamido)hexanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ BS2G-d0 (bis(sulfosuccinimidyl) glutarate-d0)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of a 7.7A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientific™ NHS-Phosphine
Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.
Thermo Scientific™ SMPH (Succinimidyl 6-((beta-maleimidopropionamido)hexanoate))
Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 12-atom (14.2A) spacer arm.
Thermo Scientific™ BMPS (N-β-maleimidopropyl-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this very short amine-to-sulfhydryl crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 5-atom spacer arm.
Thermo Scientific™ DTME (dithiobismaleimidoethane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, cleavable crosslinker composed of maleimide groups and 11-atom (13.3A) disulfide spacer arm.
![Thermo Scientific™ SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)](https://assets.fishersci.com/TFS-Assets/LSG/product-images/23013-SPB.jpg-250.jpg)
Thermo Scientific™ SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)
Crosslink amines to DNA with this crosslinker composed of NHS-ester and psoralen reactive groups; conjugates to DNA via light-activated intercalation next to pyrimidine bases.
Thermo Scientific™ Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) (sulfosuccinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ EZ-Link™ TFP Ester-PEG12-DBCO
A heterobifunctional crosslinker that contains an amine reactive tetrafluorophenyl (TFP) and a dibenzylcyclooctyne (DBCO) with a water solubilizing long PEG12 spacer in between. Use to label any primary amine containing molecule such as a protein to biomolecule with a distantly attached DBCO group.
Thermo Scientific™ SDA (NHS-Diazirine) (succinimidyl 4,4'-azipentanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ BMPH (N-β-maleimidopropionic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this short (8.1A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ AMAS (N-α-maleimidoacet-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 4-atom (4.4A) spacer arm.
Thermo Scientific™ PEG12-SPDP (PEGylated, long-chain SPDP crosslinker)
Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ L-Photo-Leucine
Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.