Protein Labeling Reagents
Abcam Rhodol, 5MG
MW 331.3 Da, Purity >95%. Highly fluorescent dye useful for labeling oligonucleotide, antibody and protein studies and for the preparation of peptide and esterase substrates. Rhodol is a structural hybrid of fluorescein and rhodamine. It retains the pH-sensitive properties conferred by a phenolic hydroxyl group, but the amine substituent reduces the pKa values. Rhodol exhibits a high extinction coefficient (~70,000 cm-1M-1) and high quantum yield (~0.8-0.9) in aqueous solution, with absorption maxima at approximately 490 nm and emission maxima between 520 and 545 nm. As with fluorescein-based dyes, the fluorescence excitation profiles of rhodol-based dyes are pH dependent, which can be useful for ratiometric pH determinations intracellularly.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Fluorescein-5-thiosemicarbazide, Fluorescent tag, 50MG
MW 421.43 g/mol, Purity >97%. Fluorescent tag for labeling of cell-surface functional groups (glycophorins). Also used as a probe for detecting protein carbonyl derivatives.
The product is subject to the following: Abcam Restricted Use Statement
Abcam 3-Dansylaminophenylboronic acid, 50MG
MW 370.2 Da, Purity >98%. Fluorescent probe for cis-diols (sugars). Labels at high pH values on cell surfaces and in glycoproteins, which is used for in vitro carbohydrate determination and HPLC analysis of sugars. Attaches to vicinal diols of sugars, oligosaccharides and certain amino alcohols forming stable cyclic complexes. This agent is also an active site inhibitor for lipases and certain serine proteases. .
The product is subject to the following: Abcam Restricted Use Statement
Abcam Pyrene 1-sulfonyl chloride, 25MG
MW 300.8 Da, Purity >85%. Amine reactive reagent for labeling peptides, proteins and other biological molecules. Fluorescence emission is environment-dependent.
The product is subject to the following: Abcam Restricted Use Statement
Sartorius Incucyte Mouse IgG1 Fabfluor-pH Antibody Labeling Dye for Antibody Internalization, 50ug vial
One vial (50 µg) of lyophilized conjugate sufficient for labeling large quantities of mouse (IgG1) test antibodies in 96-well format. Incucyte Mouse IgG1 Fabfluor-pH Red Antibody Labeling Dye utilizes a novel pH-sensitive dye coupled antibody fragment, which labels Fc-containing mouse IgG1 test antibodies and allows for rapid antibody internalization and kinetic high-throughput analysis.
STEMCELL Technologies GloCell™ Fixable Viability Dye Red 710, Size: 500 Tests
GloCell™ Fixable Viability Dye Red 710 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be used prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Red 710 is excited by the red laser (633 nm) with an excitation maximum of 647 nm, and it has a fluorescence emission maximum of 710 nm that can be detected using the 710/50 band pass filter used to detect Alexa Fluor 700.
Sigma Aldrich Fine Chemicals Biosciences Cardiogreen | 3599-32-4 | MFCD00013078 | 25mg
Cardiogreen | Mol Wt: 774.96 | 3599-32-4 | MFCD00013078 | 25mg
Aat Bioquest 6-TAMRA cadaverine
6-TAMRA cadaverine is a building block for developing fluoresceinated bioconjugates. It is also a fluorescent transglutaminase substrate to label proteins by transaminidation.
Biotium MemBrite™ Fix 488/515 Cell Surface Staining Kit, 500 Reactions
MemBrite™ Fix Cell Surface Staining Kits are designed for covalently staining the surface of live cells to conveniently visualize cell boundaries in immunofluorescence experiments. MemBrite™ Fix are new covalent cell surface stains that react irreversibly with cell surface proteins for staining that can withstand formaldehyde or alcohol fixation, and detergent permeabilization. Unlike lectins such as WGA, which bind specific targets that may vary between cell types, MemBrite™ Fix dyes react widely with cell surface proteins. MemBrite™ Fix staining is rapid and non-toxic to cells, and because MemBrite™ Fix dyes are highly water soluble, they stain cells much more evenly than traditional lipophilic membrane dyes like DiO, DiI, PKH, Vybrant, or CellMask™. This kit has reagents sufficient for 500 labeling reactions. The kit contains 5 vials of MemBrite™ Fix 488/515. Each vial is sufficient for 20 uL of 1000X dye after DMSO reconstitution.
Biotium CF488A, Aminooxy
Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. An aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF488A dye has excitation/emission at 490/515 nm.
Sigma Aldrich Fine Chemicals Biosciences Fluorescein isothiocyanate isomer I suitable for protein labeling, >=90% (HPLC), powder | 3326-32-7 | MFCD00005063 | 250MG
Fluorescein isothiocyanate isomer I suitable for protein labeling, >=90% (HPLC), powder | Purity: >=90% (HPLC) | Mol Wt: 389.38 | 3326-32-7 | MFCD00005063 | 250MG
Sigma Aldrich Fine Chemicals Biosciences 6-(2,2-Dicyanovinyl)-N-(2-hydroxyethyl)-1,2,3,4-tetrahydroquinoline | 142978-25-4 | MFCD00274418 | 5MG
6-(2,2-Dicyanovinyl)-N-(2-hydroxyethyl)-1,2,3,4-tetrahydroquinoline | Mol Wt: 253.3 | 142978-25-4 | MFCD00274418 | 5MG