Gene Reporter Systems
Promega PKCβ II Kinase Enzyme System
Recombinant full-length human PKC beta II was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC beta II is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega SYK Kinase Enzyme System
Recombinant full-length human SYK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SYK is a non-receptor protein tyrosine kinase that is widely expressed in hematopoietic cells.
Promega CDK1/CyclinA2 Kinase Enzyme System
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 is essential for completion of START, controlling event in the cell cycle required to initiate mitosis.
Promega Succinate-Glo JmjC Demethylase/Hydroxylase Assay, 10,000 assays
Succinate-Glo JmjC Demethylase/Hydroxylase Assay is a bioluminescent assay for detecting the activity of JumonjiC (JmjC) histone demethylases hydroxylases.
Promega LCK Kinase Enzyme System
Recombinant full-length human LCK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LCK (p56lck) is a member of the src family of nonreceptor tyrosine kinases.
Promega Ultra Pure UDP-Galactose, 100mM, 50microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega ZAP70 Kinase Enzyme System
Recombinant full-length human ZAP70 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAP70 is a nonreceptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor.
Promega NanoBRET TE BET BRD DNA Bundle, 1 each
NanoBRET Target Engagement Assay Components are for use with all NanoBRET Target Engagement Assays.
Promega Ultra Pure UDP-GlcNAc, 100mM, 50microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega Glo Lysis Buffer (GLB)
A proprietary formulation developed to promote rapid lysis (5 minutes) of cultured mammalian cells without scraping or freeze-thaw cycles.
Promega ONE-Glo™ Luciferase Assay System
The ONE-Glo™ Luciferase Assay System provides a sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells. Ideal for high- and ultrahigh-throughput applications.
Promega Intracellular TE Nano-Glo Substrate/Inhibitor, 10,000 assays
The Intracellular TE Nano-Glo Substrate/Inhibitor is a supplement for NanoBRET™ Target Engagement Assays.
Promega Calpain-Glo™ Assay System
The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (μ) and 2 (m) activities
Promega Tracer Dilution Buffer, 50ml
NanoBRET Target Engagement Assay Components are for use with all NanoBRET Target Engagement Assays.
Promega Luciferase Assay Systems
An extremely sensitive reagent for rapid quantitation of firefly luciferase. Can be used for assays in single-tube luminometers or in multiwell-plate luminometers with auto-injectors.