Immunohistochemistry Kits and Reagents
Abcam DoubleStain IHC kit: G&M on human tissue (BCIP & AEC), 18ML
The DoubleStain IHC kit (ab183271) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples.
The product is subject to the following: Abcam Restricted Use Statement
Invivogen Purified Lipoteichoic acid from S. aureus (LTA-SA), 5mg
Specificity: TLR2 agonist, Working concentration: 1 ng -1 μg/ml, Endotoxin level: EndoFit™, Lipoteichoic acid (LTA) is a major immunostimulatory component of Gram-positive bacteria. Like LPS, LTA is an amphiphile formed by a hydrophilic polyphosphate polymer linked to a neutral glycolipid. LTA stimulates immune cells through TLR2 to produce TNF-α and other inflammatory cytokines. Recognition of LTA involves also LPS-binding protein (LBP) and CD14.
BIOHIPPO h/m GLUT3-sh-GFP-BSD
h/m GLUT3-sh-GFP-BSD shRNA lentiviral vector expressing GFP with Blasticidin selection for studies in Human/Mouse Each vial provides a nominal titer of 5x10E6 transducing units TU Validated h/m GLUT3 shRNA Lentivirus High-titer shRNA lentiviral particles specific for human/mouse Glucose transporter 3 (GLUT3 GLUT-3 or SLC2A3) The shRNA has been validated to meet or exceed 70% GLUT3 knockdown efficiency using a specific fluorescence-based method that is more rapid and reliable than qPCR The shRNA lentivirus is ultra-purified and concentrated to high-titer by PEG precipitation and sucrose gradient centrifugation and ideal for transducing difficult-to-transfect cells including thawed and/or primary cells Order a shRNA lentivirus set and receive lentivirus produced from mix of 2 independent shRNAs validated to knockdown the target gene ( 70%) plus control lentivirus produced from mix of 2 scrambled shRNA constructs
Biocare Medical, LLC Aqua DePar, 10X
Aqua DePar is a water-soluable deparaffinization reagent which can be used for immunohistochemistry, H&E and special stains. Aqua DePar is non-flammable and safe to use. Paraffin is completely broken down and dissolved by Aqua DePar. This product eliminates the use of xylenes, xylene substitutes and alcohols, thus saving money and reducing toxic waste, hazardous and flammable materials, and exposure to child-bearing women. Hundreds of tissues and several different kinds of paraffin were tested for H&E, special stains and immunohistochemistry. A direct comparison was made with traditional xylene methods. No differences with Aqua DePar were noted between the two methods, except nuclear detail was more crisp and sharp with H&E staining with Aqua DePar compared to the traditional xylene method.
Leica Biosystems PV POLY-HRP M 110 DAB KIT
PowerVision Poly-HRP anti-Rabbit IHC Detection Systems are biotin-free, polymeric horseradish peroxidase (HRP) conjugated systems, for the manual detection of tissue-bound rabbit antibodies.
Biotium c-Myc(MYC275), CF594 conjugate, 0.1mg/mL
The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability.
Biotium Complement C4d(C4203), CF594 conjugate, 0.1mg/mL
This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, a
Abcam DoubleStain CL IHC Kit: G&Rt on human/mouse tissue (HRP/Green & AP/Red), 6ML
Abcam's Doublestain IHC Kit (ab183282) is designed to be used with user supplied goat and rat primary antibodies to detect two distinct antigens on human or mouse tissue or cell samples.
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical S-p38 MAPK InhIbItr III 10mg
A methylsulfanylimidazole that inhibits p38 MAP kinase (IC50 = 0.90 µM in vitro); potently blocks the release of TNF-α and IL-1β from human peripheral blood mononuclear cells (IC50s = 0.37 and 0.044 µM, respectively)
Abcam DoubleStain IHC Kit: G&Rt on human/mouse tissue (BCIP & AEC), 60ML
The DoubleStain IHC kit (ab183281) is designed to be used with user supplied goat and rat primary antibodies to detect two distinct antigens on human or mouse tissue or cell samples.
The product is subject to the following: Abcam Restricted Use Statement
Genetex Inc SIGNALPLUS ANTBDY ENHANCR 50ML
SignalPlus Antibody Enhancer for Western Blotting and ELISA enhances antigen-antibody reactions. SignalPlus Antibody Enhancer works well with chemiluminescence, colerimetric and fluorescence detection system. It can significantly enhance detection of weak immunoreactive and low abundance proteins in a variety of immunoassays such as Western blotting, dot blotting and ELISA. Simply dilute antibodies with SignalPlus Antibody Enhancer and process the rest of the procedures as usual. No additional steps are required. Signal enhancement is protein dependent and could vary from several folds to more than ten-fold.
Biotium CDw78(DF1588), CF594 conjugate, 0.1mg/mL
This antibody reacts with a carbohydrate determinant, identified as CDw78 (Workshop V; Code CDw78.2). Epitope recognized by this MAb and Leu21 is the same. It is present on some immature and some peripheral blood B cells, and some B cell lines. Its expression is increased after B-cell activation in vitro. CDw78 is also found on tissue macrophages and on epithelial cells, but not on T cells, NK cells, monocytes, granulocytes and myeloid tissues. It appears that CDw78 is a determinant on a major histocompatibility complex (MHC) class II subpopulation.
Medchemexpress LLC DDR1 protein, mouse, HEK293-expressed, C-terminal His-tag | >95.0% | 50 UG
Recombinant mouse Discoidin Domain Receptor 1 (DDR1) fragment (D20-T414) expressed in HEK293 cells and supplied as a C-terminal His-tagged solution in PBS. The protein is purified (>95% by reducing SDS-PAGE), 0.22 μm filtered, and provided in amounts including a 50 μg vial. Store at -80°C and ship on dry ice. Suitable for biochemical assays and antibody validation.
- Species: mouse
- Expression system: HEK293 cells
- Tag: C-terminal His
- Sequence range: D20-T414 (accession Q03146-2)
- Purity: >95% by reducing SDS-PAGE
- Formulation: 0.22 μm filtered PBS, pH 7.4, solution
- Storage: store at -80°C; shipped on dry ice
- Applications: binding assays, antibody validation, and cell signalling studies
BIOGEMS INTERNATIONAL INC Transcription Factor Fixation/Permeabilization Concentrate
The Transcription Factor Fixation/Permeabilization solution can be used with the relevant antibodies to stain for transcription factors such as Foxp3 and intranuclear staining of cytokines, chemokines, and nuclear proteins. The concentrated solution should be used with the Transcription Factor Fixation/Permeabilization Diluent (cat#92555-00) and Permeabilization Buffer (cat# 92110-00).