General Cell Based Assays
Eurofins Discoverx PathHunter eXpress UTR2 Total GPCR Internalization Assay, 10-Plate Kit. (93-0746E3CP5L)
The PathHunter UTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of UTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human GRO- ASY
The Meso Scale Discovery U-PLEX Human GRO-alpha Assay Kit is a multiplex immunoassay for measuring GRO-alpha in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection for high sensitivity and precision Requiring 50 L or less sample volume it can be automated on MSD platforms The kit includes a 96-well plate with capture and detection antibodies calibrators and controls for standard curve generation and assay validation It allows simultaneous measurement of GRO-alpha and other biomarkers with high reproducibility
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-GSK3α (Ser21) Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Phospho-GSK3α (Ser21) Detection Kit, 50,000 Assay Points
Indigo Biosciences Human Macrophage Colony-Stimulating Factor Receptor (M-CSFR) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Macrophage Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against M-CSFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Revvity Health Sciences Inc HTRF Human Phospho-RIPK1 (Ser166) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-RIPK1 (Ser166) Detection Kit, 50,000 Assay Points
Eurofins Discoverx PathHunter eXpress CXCR3 Activated GPCR Internalization Assay, 2pl Kit. (93-0661E3CP0M)
The PathHunter CXCR3 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR3 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR3. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR3-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Indigo Biosciences Human c-MET/HGFR All-inclusive Cell-Based Luciferase Reporter Assay Kit, 1x 96-well format assay
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human c-MET / Hepatocyte Growth Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against c-MET/HGFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Eurofins Discoverx PathHunter eXpress AVPR1A U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0587E3CP0M)
The PathHunter AVPR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR1A and EA-tagged β-Arrestin-2. Activation of AVPR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rDRD1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0620E2CP2M)
The PathHunter rDRD eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of rDRD GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rDRD and EA-tagged β-Arrestin-2. Activation of rDRD stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology Kallikrein 8 Rabbit mAb
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
Meso Scale Discovery U-PLEX Human IL-17D ASY
The Meso Scale Discovery U-PLEX Human IL-17D Assay Kit is a multiplex immunoassay that measures IL-17D levels in human serum plasma and cell culture supernatant samples The assay uses electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators and diluents needed to perform the assay The kit can quantitatively measure IL-17D levels with high precision and accuracy It is for research use only
Meso Scale Discovery R-PLEX Human PC9/PCSK9 ASY
The R-PLEX Human PC9/PCSK9 Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that simultaneously measures Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Progranulin (PC9) in human serum plasma and cell culture supernatants This electrochemiluminescence (ECL) based assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible quantitation of PCSK9 and PC9 levels from a single small-volume sample With a broad dynamic range and rapid protocol this assay kit allows high-throughput profiling of PCSK9 and PC9 biomarkers involved in lipid metabolism and inflammation
Bioassay Systems EnzyFluo™ Bile Acid Assay Kit. For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism.
For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range of 1 - 150 µM bile acids. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1 µM.
Eurofins Discoverx PathHunter eXpress SCTR CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0237E2CP0L)
The PathHunter SCTR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. secretin) based activation of SCTR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SCTR and EA-tagged β-Arrestin-2. Activation of SCTR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
MilliporeSigma BIOLOGY CHEMISCREEN153 CX3C
Life Sciences,Apoptosis & Cell Biology,ChemiSCREEN CX3CR1 Membrane Preparation