General Cell Based Assays
Aat Bioquest Amplite™ Colorimetric NADPH Assay Kit
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+.
Aat Bioquest ROS Brite™ 570 *Optimized for Detecting Reactive Oxygen Species (ROS)*
Reactive oxygen species (ROS) are chemically reactive molecules containing oxygen. Examples include superoxide, hydroxyl radical, singlet oxygen and peroxides. ROS is highly reactive due to the presence of unpaired valence shell electrons.
Dojindo Molecular Technologies Inc JC-1 MitoMP Detection Kit (1 set), easy to dissolve, can be detected by several instruments (Ex=500 nm, Em=530 nm), Imaging Buffer included: keeps the cell condition optimal for a long period.
Mitochondria is one of the important organelles in discussing early cytotoxicity, oxidative stress, and apoptosis in vivo. Mitochondria synthesize ATP using oxygen to produce the necessary energy for living cells. The lowering or dysfunction of mitochondrial activity is known to be closely related to cancer, aging, and neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases. JC-1 is widely used for observing mitochondrial membrane potential. JC-1 forms aggregates (in healthy mitochondria) with red fluorescence (590 nm). As membrane potential decreases, JC-1 dissociates into monomers, which show green fluorescence (530 nm).Unlike other commercially available JC-1, no precipitation of JC-1 aqueous buffer occurs by using this kit. In addition, the Image Buffer included in this kit minimizes fluorescent background and maintains cell health during assays. Dojindo offers various reagents for mitochondria research. (https://www.dojindo.com/mitochondria-research/)
Dojindo Molecular Technologies Inc Cellular Senescence Detection Kit (10 plate), detects SA-β-gal with high sensitivity by SPiDER-βGal, applicable to both living and fixed cells, by fluorescence microscopy or flow cytometry.
SPiDER-βGal is a new reagent to detect β-galactosidase specifically which possesses a high cell-permeability and a high retentivity inside cells. This novel reagent can detect β-galactosidase not only in fixed cells but also in living cells, and also can be applied to quantitative analysis by flow cytometry. Senescence-associated beta-galactosidase (SA-β-gal), which is overexpressed in senescent cells, has been widely used as a marker of cellular senescence. Cellular Senescence Detection Kit – SPiDER-βGal is optimized to detect intracellular SA-β-gal. Modern publications with a high number of citations summarize the various features of cellular senescence on the following page (https://www.dojindo.com/senescent-cell-detection-selection-guide/).
Agilent Technologies XFE96/XF PRO PDL FLUXPAK MINI
Seahorse XFe96/XF Pro PDL FluxPak Mini. For use with XFe96 and XF Pro Analyzer. Seahorse XFe96/XF Pro PDL FluxPak Mini includes 6 XFe96/XF Pro sensor cartridges, 6 XFe96/XF Pro PDL Cell Culture Microplates, and 1 bottle of Seahorse XF Calibrant Solution 500mL.
Abcam Cathepsin B Assay Kit (Magic Red)
Cathepsin B Assay Kit (Magic Red)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Phosphatidic Acid Assay Kit (Fluorometric).
Phosphatidic Acid Assay Kit (Fluorometric).
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx AssayComplete Cell Plating 16 Reagent, 100. (93-0563R16A)
AssayComplete Cell Plating 16 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress C5AR1 Activated GPCR Internalization Assay, 2pl Kit. (93-0777E3CP5M)
The PathHunter C5AR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of C5AR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged C5AR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting C5AR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCXCR7 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0689E3MCP5M)
The PathHunter mCXCR7 (CMKOR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of mCXCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR7 and EA-tagged β-Arrestin-2. Activation of mCXCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human GPR88 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR88 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR88 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Ketone Body Assay Kit
Ketone Body Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress PRLR-JAK2 Functional Assay, 2-Plate Kit. (93-0687E3CP0M)
The PathHunter PRLR-JAK2 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses PRLR activation via detection of JAK2 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged PRLR cytokine receptor, an untagged CTK JAK2 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 13 Reagent, 100. (93-0563R13A)
AssayComplete Cell Plating 13 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress KDR Activity Assay, 10-Plate Kit. (93-0903E3CP16L)
The PathHunter KDR Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activation via detection of resulting KDR phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The cell line overexpresses ProLink-tagged fusion of the KDR kinase domain with transmembrane and extracellular ligand binding domain of EGFR; and also an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.