General Cell Based Assays
Abcam Asparaginase Activity Assay Kit
Asparaginase Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Electron Microscopy Sciences Flim Sleeve 135 mm/24 exp with Clear open flap
Polyguard Polypropylene film sleeves are the finest archival quality material, and are guaranteed to give you ultimate protection for your most important film negatives. Polyguard is 100% acid free, as well as being free from optical distortion. It is resistant to water, grease, oil, alkalies, and organic solvents. Polyguard is durable, yet pliable and gentle to the film and will not become brittle, crack, or abrade the film.
Type: Clear open flap
Eurofins Discoverx InCELL Hunter eXpress BLK Kinase Binding Assay, 10pl Kit. (96-0044E1CP0L)
The InCELL Hunter eXpress BLK Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of BLK via detection of BLK levels. This product uses EFC technology. The cell line included overexpresses BLK target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human Total AKT Detection Kit, 500 Assay Points
HTRF Human Total AKT Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF HMGB1 Detection Kit, 500 Assay Points
HTRF HMGB1 Detection Kit, 500 Assay Points
Abcam Alcohol Dehydrogenase Assay Kit
Alcohol Dehydrogenase Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Cell Biolabs Inc CytoSelect™ LDH Cytotoxicity Assay Kit, 960 assays
The CytoSelect™ LDH Cytotoxicity Assay Kit provides a colorimetric format for measuring cell cytotoxicity. Cells can be plated and then treated with compounds or agents that affect cell viability. Upon cell death, lactate dehydrogenase (LDH), a soluble enzyme found in the cytoplasm, is released into the growth media. In the presence of lactate substrate, LDH converts lactate to pyruvate and generates nicotinamide adenine dinucleotide (NADH). The WST-1 molecule is converted to the orange formazan form. An increase in cell cytotoxicity is accompanied by increased LDH release and increased colorimetric signal. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues, depending on LDH expression levels. The LDH Cytotoxicity Reagent can be used to detect cytotoxicity in mammalian cells. The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates.
Eurofins Discoverx PathHunter Human ADRB2 (W158A) ENDO-EA Pharmacotrafficking Cell-Based Assay Kit (U2OS)
The PathHunter Human ADRB2 (W158A) ENDO-EA Pharmacotrafficking Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure pharmacochaperone-based rescue of the mutant ADRB2's trafficking to early endosomes. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
STEMCELL Technologies ImmunoCult™ NK Cell Expansion Coating Material, Size: 1.5 mL
Consistently expand natural killer (NK) cells in serum-free conditions without the use of problematic feeder cells.Use ImmunoCult™ NK Cell Expansion Kit to provide optimized culture conditions for expanding NK cells with high yields. This kit comprises ImmunoCult™ NK Cell Base Medium, ImmunoCult™ NK Cell Expansion Supplement, and ImmunoCult™ NK Cell Expansion Coating Material, providing you with a complete, easy-to-use culture system. After just 14 days in culture, cells can be harvested and used directly in your downstream applications.This kit is compatible with many of our other upstream and downstream products. For example, you can use EasySep™ cell separation kits to isolate NK cells that can then be immediately expanded using ImmunoCult™ NK Cell Expansion Kit.
Eurofins Discoverx PathHunter eXpress mEDNRA CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0685E2MCP0M)
The PathHunter mEDNRA eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-1) based activation of mEDNRA GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRA and EA-tagged β-Arrestin-2. Activation of mEDNRA stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human CXCL13 (CXCR5) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human CXCL13 (CXCR5) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CXCR5 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress SCTR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0237E2CP0M)
The PathHunter SCTR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. secretin) based activation of SCTR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SCTR and EA-tagged β-Arrestin-2. Activation of SCTR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human ACE2 ASY
The R-PLEX Human ACE2 Assay from Meso Scale Diagnostics is a multiplex immunoassay that measures human angiotensin-converting enzyme 2 (ACE2) levels in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The assay can help monitor ACE2 levels which may be relevant in COVID-19 infection and research This 10-plex assay includes a total of 10 analytes including human ACE2 and 9 other biomarkers
Cayman Chemical Hemoglobin Colorimetric Assay Kit, 192 Well
Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO). Cayman’s Hemoglobin Colorimetric Assay provides a quick, reliable method for determining total hemoglobin concentration in a variety of biological samples, including blood, tissue homogenates and cell lysates. Unlike the universally accepted reference method of hemoglobin determination, which uses potassium cyanide as a reagent and commonly under-estimates hemoglobin levels, Cayman’s Hemoglobin Colorimetric Assay utilizes an optimized detection reagent which is non-toxic, reporting accurate measurements of total hemoglobin concentrations with an absorbance between 560-590 nm.
Dojindo Molecular Technologies Inc NAD/NADH Assay Kit-WST (100TESTS), enables quantification of the amount of total NAD+/NADH, NAD+ and NADH in cells and measurement of their ratio.
Recently, it has become clear that Sirtuin is linked to longevity and plays a role in NAD+ level regulation. Also Sirtuin has been recognized as a marker necessary to understanding biological states, such as obesity & diabetes, as well as cellular differentiation. Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD+ levels can be determined by subtracting NADH levels from independently measured total NAD+/NADH levels. NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of their ratio. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)