General Cell Based Assays
Eurofins Discoverx PathHunter eXpress DRD2S (Short Isoform) CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0351E2CP0L)
The PathHunter DRD2S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD2S GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD2S and EA-tagged β-Arrestin-2. Activation of DRD2S stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Phagocytosis Assay Kit (Green Zymosan)
Phagocytosis Assay Kit (Green Zymosan)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter PDGFRa Bioassay Kit, 10-Plate. (93-0823Y3-00072)
The PathHunter PDGFRa Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. PDGF-BB) based activation of PDGFRa receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged PDGFRa target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam PKC Kinase Activity Assay Kit
PKC Kinase Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CXCR2 Activated GPCR Internalization Assay, 2pl Kit. (93-0934E3CP5M)
The PathHunter CXCR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CALCRL-RAMP3 CHO-K1 GPCR Assay, 2-plate Kit. (95-0169E2CP2M)
The cAMP Hunter CALCRL-RAMP3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. adrenomedullin) based activation of CALCRL (paired with RAMP3) GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCRL, a naturally Gs coupled GPCR, alongside modulator protein RAMP3. Upon activation of CALCRL, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRM1 Total GPCR Internalization Assay, 2-Plate Kit. (93-0745E3CP5M)
The PathHunter OPRM1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OPRM1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged OPRM1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRM1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices Fluorescent Assay Kit, cAMP, 96-Well, Bulk
The CatchPoint cAMP Fluorescent Assay Kit is a 96-well competitive immunoassay for quantitative measurement of intracellular or biochemical cAMP levels in cell-based and enzymatic assays. It provides high-affinity reagents and pre-coated microplates optimized for sensitive fluorescence detection.
- Format: 96-well bulk kit with reagents for ten 96-well plates.
- Target analyte: 3',5'-cyclic adenosine monophosphate (cAMP).
- Detection: fluorescent competitive immunoassay, 120 μL total assay volume per well.
- Contents: pre-coated black 96-well plates, rabbit anti-cAMP antibody, HRP-cAMP conjugate, cAMP calibrator, substrate, assay buffer, wash concentrate, cell lysis buffer.
- Incubation: 2 hours for antibody/conjugate incubation; substrate signal readable from 10 minutes up to 24 hours.
- Storage: store components at 4°C; reconstituted reagents stable for about 3 weeks at 4°C.
Cayman Chemical 8-ethylthio NAD sodium salt
A derivative of NAD+; has been used in the screening of analog-sensitive PARP1 gatekeeper mutations
Eurofins Discoverx cAMP Hunter eXpress ADRA2A CHO-K1 GPCR Assay, 10-Plate Kit. (95-0126E2CP2L)
The cAMP Hunter ADRA2A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2A, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress ADRB2 (B2AR) CHO-K1 GPCR Assay, 2-Plate Kit. (95-0121E2CP2M)
The cAMP Hunter ADRB2 (B2AR) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB2, a naturally Gs coupled GPCR. Upon activation of ADRB2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical NFAT Activation Inhibitor III, Purity: ≥98%, CAS: 3519-82-2, 5 mg
A cell-permeant inhibitor of NFAT signaling; binds calcineurin (Kd = 0.8 µM), preventing it from dephosphorylating NFAT
Meso Scale Discovery R-PLEX AB42 4G8 ASY
The Meso Scale Discovery R-PLEX Human Abeta40 6E10 Assay is a multiplex immunoassay kit for detecting amyloid beta 40 (Abeta40) and amyloid beta bound by antibody 6E10 in human samples like serum plasma and CSF It uses electrochemiluminescence (ECL) technology for high sensitivity and a broad dynamic range The assay requires 50 L or less of sample and can be completed in 3 5 hours It includes a 96-well plate with capture antibodies detection antibodies standards calibrators controls diluent read buffer wash buffer and plate seals
Eurofins Discoverx PathHunter eXpress GPR97 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0410E2ACP1M)
The PathHunter GPR97 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR97 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR97 and EA-tagged β-Arrestin-2. Activation of GPR97 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 27 Reagent, 500. (93-0563R27B)
AssayComplete Cell Plating 27 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.