General Cell Based Assays
Eurofins Discoverx PathHunter eXpress ADORA3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0586E2CP2M)
The PathHunter ADORA3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. MECA) based activation of ADORA3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADORA3 and EA-tagged β-Arrestin-2. Activation of ADORA3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress FPR1 Total Internalization Assay, 10pl Kit. (93-0873E3CP0L)
The PathHunter FPR1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of FPR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged FPR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the FPR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EphB1 Functional Assay, 2-Plate Kit. (93-0893E3CP16M)
The PathHunter eXpress EphB1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphB1 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TRIBIOSCIENCE INC Cytochrome C Reductase Activity Assay 100 Tests
NADPH-cytochrome C reductase is widely used as an endoplasmic reticulum marker and as a biomarker of ecological pollution and dietary lipid uptake. This kit is based on a colorimetric assay that measures the reduction of cytochrome C by NADPH-Cytochrome C reductase in the presence of NADPH. The reduction of cytochrome C results in the formation of distinct bands in the absorption spectrum and the increase in absorbance at 550 nm is measured with time.
Eurofins Discoverx cAMP Hunter Exenatide Bioassay Kit, 10-Plate. (95-0062Y2-00102)
The cAMP Hunter Exenatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Exenatide based activation of GLP1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx cAMP Hunter eXpress SSTR4 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0059E2CP2M)
The cAMP Hunter SSTR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of SSTR4 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type SSTR4, a naturally Gi/Go coupled GPCR. Upon activation of SSTR4, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery sAPPalpha/sAPPbeta Kit
The SappAlpha/SappBeta Kit from Meso Scale Diagnostics measures alpha and beta secretase activity in biological samples It includes reagents plates substrates detection antibodies buffers and calibrators Using electrochemiluminescent detection it offers sensitive quantification of secretase activity The kit supports high-throughput screening of compounds affecting amyloid precursor protein processing Suitable for cell or tissue extracts it requires small sample volumes and includes reagents for 96 wells processing up to 40 samples in duplicate
Eurofins Discoverx PathHunter Insulin Bioassay Kit, 10-Plate. (93-0466Y3-00008)
The PathHunter Insulin Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses insulin based activation of INSRb RTK receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress HCRTR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0258E2CP0L)
The PathHunter HCRTR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orexin A) based activation of HCRTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HCRTR1 and EA-tagged β-Arrestin-2. Activation of HCRTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693) 100 Tests
Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693) 100 Tests
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress MC5R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0231E2CP2L)
The PathHunter MC5R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-MSH) based activation of MC5R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC5R and EA-tagged β-Arrestin-2. Activation of MC5R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Echelon Biosciences Research Labs PI(3 5)P2 DiC16 1 mg
PI(3,5)P2 diC16 9Phosphatidylinositol 3,5-bisphosphate diC16) is a synthetic, purified dipalmitoyl PI(3,5)P2.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,5-bisphosphate is in very low abundance and is required for retrograde membrane trafficking from lysosomal and late endosomal compartments to the Golgi and is involved in autophagy. PI(3,5)P2 levels are controlled by the 5-kinase PIKfyve and the 5-phosphatase Fig4/Sac3 and disregulation is linked to several human neuropathies (eg Chacot-Marie-Tooth disease). This item is non returnable
Eurofins Discoverx PathHunter eXpress EDNRB Total Internalization Assay, 2pl Kit. (93-0874E3CP0M)
The PathHunter EDNRB Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of EDNRB via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged EDNRB and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the EDNRB receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter FGFR4-β-Klotho Bioassay Kit, 10pl. (93-1060Y3-00090)
The PathHunter FGFR4-β-Klotho Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses FGF19-induced activation of FGFR4-β-Klotho RTK receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, β-Klotho. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
ILEX LIFE SCIENCES LLC QuickZyme Hydroxyproline Assay Kit Colorimetric Method 1/EA
QuickZyme Hydroxyproline Assay Kit 2 x 96 Assays (includes 2x 96-well plates) The QuickZyme Hydroxyproline Assay Kit enables quantitative collagen analysis in all types of tissues in a fast and simple 96-well plate format This colorimetric assay does not need the drying step following acid hydrolysis of collagen for which often special equipment is needed This assay is the ONLY hydroxyproline-based assay that does not need this laborious step resulting in a fast sensitive and easy assay NOTE This kit does not contain tubes or a protocol for collagen hydrolysis If you need these items please order the QuickZyme Total Collagen Assay Application conditioned media cell extracts tissues tissue extracts Sensitivity 2 4 M (0 3 g/ml) Assay-time 2 hours QuickZyme Hydroxyproline Assay can be paired with QuickZyme Total Protein Assay to allow the expression of data as hydroxyproline quantity per total amount of protein