General Cell Based Assays
Eurofins Discoverx PathHunter Human NF-kB Pathway Reporter Cell-Based Assay Kit (U2OS)
The PathHunter Human NF-kB Pathway Reporter Stable Cell Line Assay (U2OS) of induced expression levels of a tagged reporter protein. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Assay Kit.
Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Assay Kit.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress HRH2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0208E2CP0L)
The PathHunter HRH2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HRH2 and EA-tagged β-Arrestin-2. Activation of HRH2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress Flt4 Activity Assay, 10-Plate Kit. (93-0888E3CP16L)
The PathHunter FLT4 Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activity via detection of resulting FLT4 phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FLT4 to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0192E2CP0M)
The PathHunter CCR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL2/MCP-1) based activation of CCR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR2 and EA-tagged β-Arrestin-2. Activation of CCR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GPR35 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0152E2CP2L)
The cAMP Hunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR35, a naturally Gi/Go coupled GPCR. Upon activation of GPR35, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc Human EXtracellular superoXide
Human EXtracellular superoXide dismutase [Cu-Zn] (SOD3) ELISA kit from CUSABIO Detection Range 7 8 pg/mL-500 pg/mL Sensitivity 1 95 pg/mL Sample serum plasma cell culture supernates tissue homogenates saliva urine Method Sandwich
Eurofins Discoverx HitHunter cAMP Assay for Biologics, 200/800 dp (96-well/384-well). (90-0075LM2)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 2 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Hampton Research HR2-137/MembFac HT - 1 ml, Deep Well block format
HR2-137/MembFac HT - 1 ml, Deep Well block format. Please note items from this supplier are non-returnable.