General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress CRHR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0048E2CP2L)
The cAMP Hunter CRHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CRH, sauvagine) based activation of CRHR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CRHR2, a naturally Gs coupled GPCR. Upon activation of CRHR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CXCR1 (IL8RA) CHO-K1 GPCR Assay, 2-Plate Kit. (95-0073E2CP2M)
The cAMP Hunter CXCR1 (IL8RA) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of CXCR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR1, a naturally Gi/Go coupled GPCR. Upon activation of CXCR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam MnTBAP chloride, Supero x ide dismutase (SOD) mimetic, 50MG
MW 879.1 Da. Cell-permeable SOD mimetic. Potent peroxynitrite scavenger. Does not scavenge NO. Shows anti-inflammatory effects and reduces oxidative injury in vivo. Reduces ROS and shows a neuroprotective effect in spinal cord injury.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress FOXO3 Nuclear Translocation Assay, 10pl Kit. (93-0876E3CP5L)
The PathHunter eXpress FOXO3 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the PI3 pathway. This kit assesses ligand (e.g. wortmannin) based pathway activation via detection of resulting FOXO3 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged FOXO3 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of FOXO3 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXGR1 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0693E2CP1M)
The PathHunter OXGR1 (GPR80, GPR99) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of OXGR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OXGR1 and EA-tagged β-Arrestin-2. Activation of OXGR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCXCR7 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0689E3MCP5M)
The PathHunter mCXCR7 (CMKOR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of mCXCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR7 and EA-tagged β-Arrestin-2. Activation of mCXCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Tag-Lite Adenosine A2A Receptor Red Antagonist Ligand, 5,000 Assay Points
HTRF Tag-Lite Adenosine A2A Receptor Red Antagonist Ligand, 5,000 Assay Points
Revvity Health Sciences Inc HTRF Human Phospho-FGFR2 (Tyr653/654) Detection Kit, 500 Assay Points
HTRF Human Phospho-FGFR2 (Tyr653/654) Detection Kit, 500 Assay Points
Cell Biolabs Inc CytoSelect™ 96-Well Cell Migration Assay Kit (3 µm, Fluorometric), 96 assays
Chemotaxis, a type of cell migration, is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. The initial response of a cell to a migration-promoting agent is to polarize and extend protrusions in the direction of the attractant; these protrusions can consist of large, broad lamellipodia or spike-like filopodia that are driven by actin polymerization and can be stabilized by extracellular matrix (ECM) adhesion or cell-cell interactions. The CytoSelect™ Cell Migration Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the migratory properties of cells. The kit contains 96 polycarbonate membrane inserts with 3 um pores in a 96-well plate, through which migratory cells are able to pass. Cells are quantified using the provided fluorometric dye. The 3 µm pore size is optimal for measuring the migration of leukocytes.
Eurofins Discoverx PathHunter eXpress rhTrkA-rhP75 Functional Assay, 2pl Kit. (93-1009E3CP16M)
The PathHunter eXpress rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the rhesus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.