General Cell Based Assays
Apexbio Technology LLC LDH Cytotoxicity Assay Kit(Synonyms: LDH Cytotoxicity Detection Kit, LDH Release Assay Kit, Lactate Dehydrogenase Cytotoxicity Assay), 200T.
The LDH Cytotoxicity Assay Kit is a biochemical assay designed to quantitatively assess cell cytotoxicity through measurement of lactate dehydrogenase (LDH) enzyme activity released into the cell culture medium LDH an intracellular enzyme ubiquitously expressed is released extracellularly upon damage or death of the cell membrane This assay relies on LDH-mediated conversion of lactate to pyruvate coupled with NAD reduction to NADH The reaction product NADH interacts with specific substrates within the kit generating a compound measurable spectrophotometrically at 490 nm Accordingly absorbance intensity at this wavelength directly correlates with cellular damage severity Compared to radioactive chromium ( 51Cr) release assays this method provides comparable quantitative data in a safer non-radioactive format applicable for cell death cytotoxicity and drug screening research
Apexbio Technology LLC LDH Cytotoxicity Assay Kit(Synonyms: LDH Cytotoxicity Detection Kit, LDH Release Assay Kit, Lactate Dehydrogenase Cytotoxicity Assay), 1000T.
The LDH Cytotoxicity Assay Kit is a biochemical assay designed to quantitatively assess cell cytotoxicity through measurement of lactate dehydrogenase (LDH) enzyme activity released into the cell culture medium LDH an intracellular enzyme ubiquitously expressed is released extracellularly upon damage or death of the cell membrane This assay relies on LDH-mediated conversion of lactate to pyruvate coupled with NAD reduction to NADH The reaction product NADH interacts with specific substrates within the kit generating a compound measurable spectrophotometrically at 490 nm Accordingly absorbance intensity at this wavelength directly correlates with cellular damage severity Compared to radioactive chromium ( 51Cr) release assays this method provides comparable quantitative data in a safer non-radioactive format applicable for cell death cytotoxicity and drug screening research
ABclonal Technology β-Actin Rabbit pAb
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Meso Scale Discovery sAPPalpha/sAPPbeta Kit
The SappAlpha/SappBeta Kit from Meso Scale Diagnostics measures alpha and beta secretase activity in biological samples It includes reagents plates substrates detection antibodies buffers and calibrators Using electrochemiluminescent detection it offers sensitive quantification of secretase activity The kit supports high-throughput screening of compounds affecting amyloid precursor protein processing Suitable for cell or tissue extracts it requires small sample volumes and includes reagents for 96 wells processing up to 40 samples in duplicate
Meso Scale Discovery R-PLEX Human GDF-8 ASY
The R-PLEX Human GDF-8 Assay is a multiplex immunoassay kit from Meso Scale Diagnostics that allows for the quantitative measurement of GDF-8 (also known as myostatin) in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay can be run on Meso Scale Diagnostic s SECTOR Imagers and QuickPlex plate readers It includes a plate coated with capture antibodies detection antibodies calibrators and reagents required for the assay
Meso Scale Discovery U-PLEX Human MMP-9 total ASY
The Meso Scale Discovery U-PLEX Human MMP-9 Total Assay is an electrochemiluminescent immunoassay for the quantitative measurement of total MMP-9 in human serum plasma and cell culture supernatants It utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and rapid detection of MMP-9 levels down to 0 04 ng/mL The assay requires a small sample volume of 50 L and has a 3 5 hour total assay time The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to MSD SULFO-TAG calibrator and sample diluent It provides a simple reproducible method to quantify MMP-9 levels for research use
Meso Scale Discovery U-PLEX Mouse IL-9 ASY
The Meso Scale Discovery U-PLEX Mouse IL-9 Assay Kit is an electrochemiluminescent immunoassay for the quantitative measurement of mouse interleukin 9 (IL-9) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible quantitation of mouse IL-9 with a lower limit of detection of 0 3 pg/mL and an upper limit of 10 000 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to MSD SULFO-TAG calibrators and sample diluent The assay takes approximately 3 5 hours to complete after a 2 hour incubation
Amsbio LLC Oris Cell Migration Assay - Fibronectin Coated
Oris™ Cell Migration Assay - Fibronectin Coated
Amsbio LLC Sulfated Glycosaminoglycan Quantification Kit (Proteoglycan Detection)
Sulfated Glycosaminoglycan Quantification Kit (Proteoglycan Detection)
Meso Scale Discovery U-PLEX Mouse IL-4 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-4 Assay Kit is an electrochemiluminescent immunoassay for quantifying mouse interleukin 4 (IL-4) in various samples It uses MSD s SECTOR Imager to detect signals from microplate wells The kit includes a capture antibody for IL-4 and a ruthenium-labeled detection antibody It has a detection range of 0 05-1000 pg/mL with no cross-reactivity with other cytokines The kit provides a 96-well plate detection antibody calibrators 10X diluent and components for 96 tests
Meso Scale Discovery U-PLEX Human IL-17A ASY
The Meso Scale Discovery U-PLEX Human IL-17A Assay Kit is an electrochemiluminescent immunoassay for the quantitative measurement of IL-17A in human serum plasma and cell culture supernatants The assay uses MSD s proprietary SECTOR Imager and provides a sensitive and rapid method for detecting IL-17A with a lower limit of detection of 0 04 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to an electrochemiluminescent label calibrators and sample diluent The assay takes approximately 3 5 hours to complete
Meso Scale Discovery RPLEX MSE Osteoprotegerin ASY
The Meso Scale Discovery R-PLEX Mouse Osteoprotegerin Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of mouse osteoprotegerin (OPG) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The assay has a lower limit of detection of 0 04 ng/mL for mouse OPG and a typical assay time of 3 5 hours This assay enables researchers to accurately quantify circulating OPG levels in mouse models