General Cell Based Assays
Eurofins Discoverx PathHunter eXpress P2RY1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0941E3CP5L)
The PathHunter P2RY1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. ATP) based activation of P2RY1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY1 and EA-tagged β-Arrestin-2. Activation of P2RY1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mEBI2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0953E2MCP0L)
The PathHunter mEBI2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dihydroxycholesterol) based activation of mEBI2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEBI2 and EA-tagged β-Arrestin-2. Activation of mEBI2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CX3CR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0956E2CP0L)
The PathHunter CX3CR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CX3CL1/fractalkine) based activation of CX3CR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CX3CR1 and EA-tagged β-Arrestin-2. Activation of CX3CR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVRL1/ACVR2B Dimerization Assay, 10pl Kit. (93-0964E3CP22L)
The PathHunter ACVRL1/ACVR2B Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/ACVR2B receptors. This product uses EFC technology. The cell line included overexpresses ACVRL1 target protein fused to PK alongside ACVR2B target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/ACVR2B, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Lipid Staining Kit
Lipid Staining Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress FGFR1 Functional Assay, 10-Plate Kit. (93-1090E3CP16L)
The PathHunter eXpress FGFR1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FGFR1 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioss Glutathione Reductase Monoclon
Glutathione Reductase Primary Antibody Unconjugated Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB FCM RecombinantConcentration 1ug/ul Size 100 ul Storage Buffer 10mM phosphate buffered saline(pH 7 4) with 150mM sodium chloride 0 05% BSA 0 02% sodium azide and 50% glycerol
Eurofins Discoverx PathHunter eXpress rHTR2C U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0915E3RCP0L)
The PathHunter rHTR2C eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of rHTR2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rHTR2C and EA-tagged β-Arrestin-2. Activation of rHTR2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rGPR35 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0887E2RCP2L)
The PathHunter rGPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of rGPR35 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rGPR35 and EA-tagged β-Arrestin-2. Activation of rGPR35 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
AdipoGen Isthmin-1 human ELISA Kit
Colorimetric Sandwich ELISA Assay. Detects human Isthmin-1 in plasma and cell culture supernatant. It detects also mouse Isthmin-1 very conserved. Range 0.625 to 40ng/ml. Sensitivity 0.4 ng/ml. Samples Cell Culture Supernatant, Plasma. Isthmin-1 ISM1 was first identified as a gene expressed in the Xenopus midbrain-hindbrain organizer called the isthmus, with a proposed role during early brain development. Isthmin-1 encodes a predicted ~50-kDa protein containing a signal peptide, a thrombospondin domain and an adhesion-associated domain. Isthmin-1 is important for embryonic and postnatal development. Growing evidence has shown that aberrant expression of Isthmin-1 can also affect the biological behavior of cancer. The Ism1 gene is conserved in mice and humans. A recent study showed that Isthmin-1 is an adipokine that induces glucose uptake in human and mouse adipocytes.
AdipoGen Insulin human ELISA Kit
Colorimetric Sandwich ELISA Assay. Detects human Insulin in serum, plasma and cell culture supernatant. Range 7.81 to 500pg/ml. Sensitivity 10 pg/ml. Samples Cell Culture Supernatant, Plasma, Serum. Glucose is the main fuel for the human body and blood glucose values are tightly regulated in healthy individuals. Regulation of glucose uptake in cells is performed by the hormone called insulin which is glucose-responsively secreted from pancreatic beta cells. When the glucose concentration in the blood is increased, insulin lowers it by increasing glucose uptake by muscle, liver and fat cells. Insulin is synthesized by beta cells of the pancreas in the form of a single chain of three peptides B, C and A in the order B-chain-C peptide-A-chain. The mature insulin consists of B-chain 30 amino acids and A-chain 21 amino acids linked by two inter-chain and one intra-chain disulfide bridge.