General Cell Based Assays
Cayman Chemical ProteIn SynthesIs asy KIt 96
Cayman’s Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to cells, the OPP probe incorporates into the C-terminus of translating polypeptide chains, thereby stopping translation. These truncated C-terminal alkyne-labeled proteins are then subsequently detected via copper-catalyzed click chemistry using 5 FAM-Azide. The reagents provided in the kit are sufficient to run 20 samples when using flow cytometry or 100 samples when using a 96-well plate format.
Cayman Chemical Ascorbate asy Buffer 25mL
A glutaminyl cyclase inhibitor (IC50 53 nM) reduces polyglutamine aggregation in HeLa cells expressing EGFP-HTT-Q74 at 12.5 and 25 UM reduces polyglutamine aggregation in primary mouse neurons expressing EGFP-Q80 at 50 UM
Cayman Chemical cPLA2 asy Buffer 1ea
An SHP-2 inhibitor (IC50 E76K (IC50 0.17 UM) reduces the levels of phosphorylated ERK in a reporter assay using KYSE-520 cancer cells (IC90 0.012 UM) and inhibits proliferation of the same cells (IC50 0.32 UM) reduces tumor volume in KYSE-520 and NCI H358 mouse xenograft models at 30 or 100 mg/kg two times per day
Cayman Chemical LPO asy FTS Reagent 2 1ea
A medium-chain acyl CoA inhibitsbutyryl-CoAacetate-CoA transferase isolated from P. elsdenii at 200 UM levels are increased in primary fibroblasts derived from patients with MCAD deficiency in the presence of heptanoic acid blocks berberine-induced decreases in infarct volume and improvements in motor function in a mouse model of MCAO-induced ischemic stroke at 0.4 mg/kg per day
Cayman Chemical Urease asy Reagent 1ea
A sesquiterpenoid with diverse biological activities selectively RXRB over RXRa and RXRY in reporter assays using HEK293T cells (EC50s 5.2 27 and 43 UM respectively) as well as RARs PPARs LXRs FXR CAR VDR and PXR at 30 UM enhances GABA-induced activation of a1B2Y2-subunit containing GABA receptors in CHO cells (EC50 12.6 UM) cytotoxic to GLC-4 and COLO 320 cells (IC50s 127 and 124 UM respectively) decreases motor performance in the rotarod test and increases pentobarbital-induced sleeping time in mice at 100 mg/kg i.p. increases the number of entries into and percentage of time spent in the open arms of the elevated plus maze in mice indicating anxiolytic-like activity at 12 mg/kg
Cayman Chemical cGAS ReactIon Buffer2X 6mL
cGAS Reaction Buffer (2X) has been tested and formulated to work exclusively with Cayman’s cGAS Inhibitor Screening Assay Kit (Item No. 701930).
Abcam PKC Kinase Activity Assay Kit
PKC Kinase Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Aat Bioquest iFluor™ 790 hydrazide
In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals.
Eurofins Discoverx PathHunter eXpress CCR8 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0196E2CP0M)
The PathHunter CCR8 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL1) based activation of CCR8 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR8 and EA-tagged β-Arrestin-2. Activation of CCR8 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
