General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GALR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0228E2CP0L)
The PathHunter GALR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GALR1 and EA-tagged β-Arrestin-2. Activation of GALR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVRL1/ACVR2B Dimerization Assay, 2pl Kit. (93-0964E3CP22M)
The PathHunter ACVRL1/ACVR2B Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/ACVR2B receptors. This product uses EFC technology. The cell line included overexpresses ACVRL1 target protein fused to PK alongside ACVR2B target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/ACVR2B, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Ko-143, BCRP inhibitor, 5MG
MW 469.6 Da. Potent, selective BCRP inhibitor (EC₅₀ values are 23 and 26 nM respectively for the export of mitoxantrone (ab145668, ab141041) and topotecan (ab120388) in cancer cell lines). Non-toxic fumitremorgin C (ab144258) analog. Shows chemopotentiating effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Indigo Biosciences INDIGO BIOSCIENCES INC Human TrkA Reporter Assay Kit, 384-well Format, Human
This all-inclusive cell-based luciferase reporter assay kit targets Human Tropomyosin receptor kinases A (TrkA/NTRK1). It uses proprietary mammalian cells engineered for constitutive TrkA expression. The kit includes TrkA Reporter Cells, optimized media, a reference activator, Luciferase Detection Reagent, and a cell culture-ready assay plate. It quantifies functional activity (activating or inhibitory) against human TrkA or the coupled Ca+2·calcineurin / NFAT pathway. Provides clear, reproducible results, exceptional cell viability post-thaw, and consistent lot-to-lot results. Store at –80°C; do not refreeze or maintain reporter cells in extended culture.
- Clear, reproducible results
- All-inclusive assay systems
- Exceptional cell viability post-thaw
- Consistent results lot to lot
Meso Scale Discovery R-PLEX Human BMP-5 ASY
The R-PLEX Human BMP-5 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures BMP-5 levels in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator diluent and lyophilized BMP-5 calibrator The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL It can be used to measure BMP-5 levels in biological samples for research applications
Meso Scale Discovery U-PLEX Rat Leptin ASY
The Meso Scale Discovery U-PLEX Rat Leptin Assay Kit is a multiplex immunoassay that measures leptin levels in rat serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with ruthenium calibrators and controls The assay requires a small sample volume and provides results in as little as 3 5 hours With minimal hands-on time this kit allows for the rapid accurate quantification of rat leptin
Cayman Chemical LysoBrIteBlue 500test
A lysosomal dye; ex/em = 353/442 nm; has been used to monitor endosomal escape of doxorubicin theranostic nanoparticles in HeLa cells
Eurofins Discoverx AssayComplete Thawing Reagent T8, 100. (92-4108TR)
AssayComplete Thawing Reagent T8 is an optimized thawing reagent for revival and recovery of Eurofins DiscoverX cell lines. This reagent ensures optimal post-thaw viability, recovery and assay performance upon thawing. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended thawing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress CCR10 Activated GPCR Internalization Assay, 10pl Kit. (93-0783E3CP5L)
The PathHunter CCR10 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR10 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR10. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR10-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.