General Cell Based Assays
Eurofins Discoverx PathHunter eXpress OPN5 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0704E2CP1L)
The PathHunter OPN5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of OPN5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPN5 and EA-tagged β-Arrestin-2. Activation of OPN5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGFR Total Internalization Assay, 10pl Kit. (93-0872E3CP0L)
The PathHunter PTGFR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGFR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGFR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGFR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress A549 G9a Methyltransferase Assay, 2-plate Kit. (96-0003E15CP7M)
The InCELL Hunter eXpress A549 G9a Methyltransferase Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of G9a via detection of G9a levels. This product uses EFC technology. The cell line included overexpresses G9a target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRD2(1) Bromodomain Assay, 10-plate Kit. (96-0004E1CP0L)
The InCELL Hunter eXpress HEK 293 BRD2(1) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD2(1) via detection of BRD2(1) levels. This product uses EFC technology. The cell line included overexpresses BRD2(1) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human S100A8/MRP8 ASY
The R-PLEX Human S100A8 (MRP8) Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that measures S100A8 protein levels in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer sample diluent and calibrators and controls The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL It is for research use only
Abcam HDAC6 Activity Assay Kit (Fluorometric)
HDAC6 Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx cAMP Hunter eXpress SSTR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0095E2CP2L)
The cAMP Hunter SSTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of SSTR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type SSTR2, a naturally Gi/Go coupled GPCR. Upon activation of SSTR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress PTHR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0118E2CP2M)
The cAMP Hunter PTHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. parathyroid hormone) based activation of PTHR1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR1, a naturally Gs coupled GPCR. Upon activation of PTHR1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress SSTR2 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0095E2CP2M)
The cAMP Hunter SSTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of SSTR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type SSTR2, a naturally Gi/Go coupled GPCR. Upon activation of SSTR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Cell Cycle Assay Kit (Fluorometric - Green)
Cell Cycle Assay Kit (Fluorometric - Green)
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical 12-Lipoxygenase leukocyte-ty
Immunogen: Full length recombinant mouse 12-lipoxygenase (leukocyte-type) • Host: Rabbit • Species Reactivity: (+) Mouse, porcine • Applications: ELISA, WB • MW = ~73 kDa
Meso Scale Discovery U-PLEX Human IL-17D ASY
The Meso Scale Discovery U-PLEX Human IL-17D Assay Kit is a multiplex immunoassay that measures IL-17D levels in human serum plasma and cell culture supernatant samples The assay uses electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent labels calibrators and diluents needed to perform the assay The kit can quantitatively measure IL-17D levels with high precision and accuracy It is for research use only