General Cell Based Assays
Bioassay Systems EnzyChrom™ Ascorbic Acid Assay Kit. For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.
For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism. Key Features: Use 20 µL samples. Linear detection range: colorimetric assay 6 to 1000 µM, fluorimetric assay 1 to 100 µM ascorbic acid. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, tissue, and cell culture. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: OD, FL: 6, 1 µM.
Bioassay Systems QuantiChrom™ Acetylcholinesterase Assay Kit. For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors.
For quantitative determination of acetylcholinesterase activity and evaluation of acetylcholinesterase inhibitors. Key Features: Sensitive and accurate. Detection range 10 to 600 U/L AChE activity in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at 2 min and 10 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD412nm. Samples: Blood, serum, plasma etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 10 U/L.
Eurofins Discoverx cAMP Hunter eXpress GPBAR1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0049E2CP2L)
The cAMP Hunter GPBAR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPBAR1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPBAR1, a naturally Gs coupled GPCR. Upon activation of GPBAR1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Lipolysis Assay Kit (Colorimetric)
Lipolysis Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Cell Migration/Chemotaxis Assay Kit (96-well, 3 ¿m)
Cell Migration/Chemotaxis Assay Kit (96-well, 3 ¿m)
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery U-PLEX Human IL-17A/F ASY
The Meso Scale Discovery U-PLEX Human IL-17A/F Assay Kit is a multiplex immunoassay that measures human interleukin 17A and interleukin 17F in cell culture supernatants serum plasma and other biological samples This electrochemiluminescent assay utilizes MSD s proprietary SECTOR Imager and provides high sensitivity a broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibodies detection antibodies Read Buffer Diluent 2 Calibrator Blend and Wash Buffer It can detect IL-17A in a range of 0 06-1500 pg/mL and IL-17F in a range of 0 10-1500 pg/mL
Eurofins Discoverx cAMP Hunter eXpress GHRHR CHO-K1 GPCR Assay, 2-Plate Kit. (95-0076E2CP2M)
The cAMP Hunter GHRHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GRF) based activation of GHRHR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GHRHR, a naturally Gs coupled GPCR. Upon activation of GHRHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Dojindo Molecular Technologies Inc Liperfluo(50UGx5) is used to monitor lipid peroxidation in ferroptosis research, emitting intense fluorescence by a lipid peroxide specific oxidation, and is applicable for microscopy and FCM analysis
Liperfluo is designed and exclusively developed by Dojindo for detection of lipid peroxides. Among fluorescent probes that detect Reactive Oxygen Species (ROS), Liperfluo is the only compound that can specifically detect lipid peroxides. Since the excitation and emission wavelengths of the oxidized Liperfluo are 524 nm and 535 nm, respectively, both photo-damage and auto-fluorescence can be minimized. The tetraethyleneglycol group linked to one end of the diisoquinoline ring helps its solubility and dispersibility to aqueous buffer. Though Liperfluo's oxidized form is almost nonfluorescent in an aqueous media, it emits fluorescence in lipophilic sites such as cell membranes. Therefore, it can easily be applied to lipid peroxide imaging by a fluorescence microscopy and a flow cytometric analysis for living cells. Liperfluo is widely used in ferroptosis research, a research field Dojindo has a strong portfolio in. (https://www.dojindo.com/ferroptosis-detection-selection-guide/)
Dojindo Molecular Technologies Inc SOD Assay Kit-WST (500 TESTS) is a colorimetric microplate and low background noise measurement which measures 100% inhibition by SOD.
Superoxide dismutase (SOD) is a crucial antioxidative enzyme, which catalyzes the dismutation of the superoxide anion (O2-) into hydrogen peroxide and molecular oxygen. In order to determine SOD activity, several direct and indirect methods have been developed. Among these methods, the indirect method using nitroblue tetrazolium (NBT) is commonly used due to the convenience and ease to use. However, using the NBT method yields poor water solubility of the formazan dye and the interaction with the reduced form of xanthine oxidase. SOD Assay Kit-WST allows for very convenient SOD assays by utilizing Dojindo's highly water-soluble tetrazolium salt, WST-1, producing a water-soluble formazan dye upon reduction with the superoxide anion. The rate of the reduction of WST-1 with O2- is linearly related to the xanthine oxidase (XO) activity depending on SOD. Therefore, the IC50 (50% inhibition activity of SOD or SOD-like materials) can be determined by the colorimetric method.
Aat Bioquest Amplite™ Colorimetric Aspartate Aminotransferase (AST) Assay Kit
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
Abcam RG 108, non-nucleoside DNA methyltransferase inhibitor (DNMT), 10MG
MW 334.3 Da, Purity >99%. Novel, non-nucleoside DNA methyltransferase (DNMT) inhibitor, capable of interacting with the DNMT1 active site (IC₅₀ = 115 nM). Inhibits DNA methylation in human cancer cell lines. Reactivates several epigenetically silenced tumour suppressor genes in vitro. Capable of enhancing the efficiency of induced pluripotent stem cell (iPS) generation.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx cAMP Hunter Exenatide Bioassay Kit, 2-Plate. (95-0062Y2-00101)
The cAMP Hunter Exenatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Exenatide based activation of GLP1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eagle Biosciences Inc Nitric Oxide Synthase Non-Enzymatic Assay Refill Kit
Nitric Oxide Synthase Non-Enzymatic Assay Refill Kit