General Cell Based Assays
Meso Scale Discovery U-PLEX Mouse IL-31 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-31 Assay Kit is a multiplex immunoassay that measures mouse interleukin 31 (IL-31) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The kit includes a plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrator diluent wash buffer read buffer and a vial of lyophilized calibrator that must be reconstituted before use The assay can quantify mouse IL-31 concentrations between 1 5 and 10 000 pg/mL
BioVendor, LLC Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Large Economy Kit Size: 400 assays), Multispecies colorimetric assay, Biocolor Item # S6000
Sircol; INSOLUBLE Collagen Assay, Bovine Collagen (Large Economy Kit Size: 400 assays). The Sircol; INSOLUBLE Collagen Assay is a dye-binding method for analysis of insoluble collagen fibers. The assay be used to assess the rate of production of newly laid down collagen fibers during periods of rapid growth, development, tissue repair, remodeling and wound healing. Sources of material includes tissues, bone and calcified tissue. It replaces the conventional hydroxyproline analysis. No more working with concentrated acids or alkalis! The Sircol Insoluble Assay uses mild acid and temperature treatment for 2 to 3 hrs. The solubility conversion to denatured collagen and subsequent measurement can be completed within one working day. The ratio of soluble and insoluble collagens can be measured using the same Sircol Dye Reagent. Multispecies colorimetric assay. Detection Limit: 10.0 g. Assay-time: 4 hours. Biocolor Item # S6000
Abcam DPPH Antioxidant Assay Kit (Colorimetric).
DPPH Antioxidant Assay Kit (Colorimetric).
The product is subject to the following: Abcam Restricted Use Statement
Abcam Hydrogen Peroxide Assay Kit
Hydrogen Peroxide Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Abcam Cathepsin B Activity Assay Kit (Fluorometric)
Cathepsin B Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Cell Biolabs Inc CytoSelect™ 96-Well Anoikis Assay Kit (Colorimetri/Fluorometric)
Adhesion to the extracellular matrix is essential for the survival of many adherent cells. Apoptosis that results from the loss of cell adhesion to the ECM, or inappropriate adhesion, is defined as “anoikis”. A common feature of carcinoma development is the ability of transformed cells to survive under anchorage independent or “spheroid” growth conditions. Resistance to anoikis has been shown to be involved in the loss of cell homeostasis, cancer growth, and metastasis. Preventing anoikis is a major goal in the development of cell transplantation techniques, including the therapeutic use of progenitor cells. The CytoSelect™ 96-well Anoikis Assay Kit provides a colorimetric and fluorometric format to measure anchorage-independent growth and monitoring anoikis propelled cell death. The kit contains sufficient reagents for 96 samples in a hydrogel-coated 96-well plate. Live cells are detected with MTT or Calcein AM. Cell death is detected with the Ethidium Homodimer (EthD-1).
Hach Company SC4500 Ctrl Prog Prof DP 1 mA
SC4500 Controller-Prognosys-Profibus DP-1 mA Input-100-240 VAC-without power cordIncludes Controller-Non-Claros Embedded Prognosys-without power cord includes mounting hardware
Abcam RG 108, non-nucleoside DNA methyltransferase inhibitor (DNMT), 10MG
MW 334.3 Da, Purity >99%. Novel, non-nucleoside DNA methyltransferase (DNMT) inhibitor, capable of interacting with the DNMT1 active site (IC₅₀ = 115 nM). Inhibits DNA methylation in human cancer cell lines. Reactivates several epigenetically silenced tumour suppressor genes in vitro. Capable of enhancing the efficiency of induced pluripotent stem cell (iPS) generation.
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc Liperfluo(50UGx5) is used to monitor lipid peroxidation in ferroptosis research, emitting intense fluorescence by a lipid peroxide specific oxidation, and is applicable for microscopy and FCM analysis
Liperfluo is designed and exclusively developed by Dojindo for detection of lipid peroxides. Among fluorescent probes that detect Reactive Oxygen Species (ROS), Liperfluo is the only compound that can specifically detect lipid peroxides. Since the excitation and emission wavelengths of the oxidized Liperfluo are 524 nm and 535 nm, respectively, both photo-damage and auto-fluorescence can be minimized. The tetraethyleneglycol group linked to one end of the diisoquinoline ring helps its solubility and dispersibility to aqueous buffer. Though Liperfluo's oxidized form is almost nonfluorescent in an aqueous media, it emits fluorescence in lipophilic sites such as cell membranes. Therefore, it can easily be applied to lipid peroxide imaging by a fluorescence microscopy and a flow cytometric analysis for living cells. Liperfluo is widely used in ferroptosis research, a research field Dojindo has a strong portfolio in. (https://www.dojindo.com/ferroptosis-detection-selection-guide/)
Dojindo Molecular Technologies Inc SOD Assay Kit-WST (500 TESTS) is a colorimetric microplate and low background noise measurement which measures 100% inhibition by SOD.
Superoxide dismutase (SOD) is a crucial antioxidative enzyme, which catalyzes the dismutation of the superoxide anion (O2-) into hydrogen peroxide and molecular oxygen. In order to determine SOD activity, several direct and indirect methods have been developed. Among these methods, the indirect method using nitroblue tetrazolium (NBT) is commonly used due to the convenience and ease to use. However, using the NBT method yields poor water solubility of the formazan dye and the interaction with the reduced form of xanthine oxidase. SOD Assay Kit-WST allows for very convenient SOD assays by utilizing Dojindo's highly water-soluble tetrazolium salt, WST-1, producing a water-soluble formazan dye upon reduction with the superoxide anion. The rate of the reduction of WST-1 with O2- is linearly related to the xanthine oxidase (XO) activity depending on SOD. Therefore, the IC50 (50% inhibition activity of SOD or SOD-like materials) can be determined by the colorimetric method.
Enzo Life Sciences GOLGI ID Green assay kit (1x1Kit)
Dependably localizes to Golgi apparatus with minimal staining of ER. Applications: Fluorescence microscopy. Contents: GOLGI ID Green detection reagent: 50nmoles. Hoechst 33342 nuclear stain: 50μl. 10X assay buffer 1: 15ml. 50X assay buffer 2: 1.2ml
Bioassay Systems QuantiChrom™ Boron Assay Kit. Quantitative determination of boron in agricultural and environmental samples.
For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm.
Aat Bioquest Amplite™ Colorimetric Aspartate Aminotransferase (AST) Assay Kit
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
Cell Biolabs Inc OxiSelect™ Comet Assay Kit (3-Well Slides), 75 assays (25 slides)
The comet assay, or single cell gel electrophoresis assay (SCGE), is a common technique for measurement of DNA damage in individual cells. Under an electrophoretic field, damaged cellular DNA (containing fragments and strand breaks) is separated from intact DNA, yielding a classic “comet tail” shape under the microscope. The extent of DNA damage is usually visually estimated by comet tail measurement; however, image analysis software is also available for measuring various parameters. The OxiSelect™ Comet Assay is a fast and sensitive kit for the measurement of cellular DNA damage. Each kit provides sufficient reagents to perform up to 25 assays (25 slides with 3 wells each).