General Cell Based Assays
Eurofins Discoverx PathHunter eXpress SSTR5 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0402E3CP0M)
The PathHunter SSTR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR5 and EA-tagged β-Arrestin-2. Activation of SSTR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress OPRL1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0115E2CP2L)
The cAMP Hunter OPRL1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. orphanin FQ) based activation of OPRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRL1, a naturally Gi/Go coupled GPCR. Upon activation of OPRL1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR12 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0329E2ACP1M)
The PathHunter GPR12 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR12 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR12 and EA-tagged β-Arrestin-2. Activation of GPR12 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 11 Reagent, 100. (93-0563R11A)
AssayComplete Cell Plating 11 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress ADORA3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0586E2CP2L)
The PathHunter ADORA3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. MECA) based activation of ADORA3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADORA3 and EA-tagged β-Arrestin-2. Activation of ADORA3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress NPFFR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0097E2CP2M)
The cAMP Hunter NPFFR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptide FF, RFRP-3) based activation of NPFFR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type NPFFR1, a naturally Gi/Go coupled GPCR. Upon activation of NPFFR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human AXL/UFO ASY
The R-PLEX Human AXL UFO Assay from Meso Scale Diagnostics is a multiplex immunoassay kit that allows for the simultaneous quantitative measurement of AXL in human serum plasma and cell culture supernatants It utilizes Meso Scale Diagnostics electrochemiluminescent detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator diluent and lyophilized calibrator It can test up to 80 samples in duplicate and has a testing time of 3 5 hours
Meso Scale Discovery U-PLEX Mouse IL-17C ASY
The Meso Scale Discovery U-PLEX Mouse IL-17C Assay is a multiplex immunoassay kit that enables simultaneous quantification of mouse interleukin 17C (IL-17C) in serum plasma and cell culture supernatants The assay utilizes MSD s proprietary electrochemiluminescent detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay allows for the accurate and reliable measurement of IL-17C which plays an important role in mucosal immunity and inflammation With minimal hands-on time required this kit serves as a useful tool for mouse IL-17C detection in research studies involving immunology and inflammation
Eurofins Discoverx PathHunter eXpress GPR132 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0411E2ACP1L)
The PathHunter GPR132 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR132 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR132 and EA-tagged β-Arrestin-2. Activation of GPR132 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVRL1/ACVR2B Dimerization Assay, 10pl Kit. (93-0964E3CP22L)
The PathHunter ACVRL1/ACVR2B Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/ACVR2B receptors. This product uses EFC technology. The cell line included overexpresses ACVRL1 target protein fused to PK alongside ACVR2B target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/ACVR2B, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR9 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0296E2CP0L)
The PathHunter CCR9 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL25) based activation of CCR9 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR9 and EA-tagged β-Arrestin-2. Activation of CCR9 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCXCR3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0553E2MCP0L)
The PathHunter mCXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of mCXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR3 and EA-tagged β-Arrestin-2. Activation of mCXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CNR2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0183E2CP2L)
The cAMP Hunter CNR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of CNR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CNR2, a naturally Gi/Go coupled GPCR. Upon activation of CNR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IL6RA/6ST Bioassay Kit, 10-Plate. (93-1045Y3-00044)
The PathHunter IL6RA/IL6ST Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-6-induced dimerization of IL6RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL6R target protein fused to EA. Upon ligand-induced dimerization of IL6RA/IL6ST, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Innovative Research Inc Hmn uPA Rcmb LMW 100ug
Human Urokinase (uPA) Recombinant Low Molecular Weight 100ug - Human Urokinase (uPA) Recombinant Low Molecular Weight from Innovative Research has been recombinantly produced in insect cells and purified using a combination of conventional and affinity chromatography. This is a frozen liquid buffered in 0.05M Sodium Acetate; 0.1M NaCl; 1mM EDTA; pH 5.0 and a purity of 95%. It is useful as a positive control for ELISA and western blot and and other applications including cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis. Purified from auto-catalytic digestion of recombinant human urokinase produced in insect cells. Active two-chain low molecular weight human urokinase contains the proteinase domain and is separated from the amino terminal fragment by cleavage at the Lys135-Lys136 bond.
