General Cell Based Assays
Genscript Corporation CHO-K1/Ga15/AMY3 Stable Cell
Receptor activity-modifying proteins (RAMPs) are a class of protein which interact with and modulate the activities of several Class B G Protein-Coupled Receptors including the receptors for secretin calcitonin (CT) glucagon and vasoactive intestinal peptide (VIP) There are three distinct types of RAMPs designated RAMP1 RAMP2 and RAMP3 each encoded by a separate gene Currently the function of RAMPs is divided into 2 class activities Association of RAMPs with either the CT or CALCRL proteins forms 6 different receptors from the calcitonin receptor family When associated with the Calcitonin receptor (CTR) or Calcitonin receptor-like (CALCRL) RAMPs can change the selectivity of the receptor for a specific hormone In the cases of the other receptors mentioned however there is no evidence that they can do this but instead function to regulate trafficking of receptors from the ER / golgi to the membrane GenScript s cloned human AMY3 expressing cell line is generated in the C
Electron Microscopy Sciences Flim Sleeve 135 mm/36 exp with Frosted Open Flap
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Zen Bio Inc SERUM TRIGLYCERIDE ASSAY KIT
Serum Triglyceride Assay Kit, 100 point assay (SINGLE PLATE)
Aat Bioquest Amplite™ Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*
Glutamic acid is one of the 20 proteinogenic amino acids. The carboxylate anions and salts of glutamic acid are known as glutamates.
Eurofins Discoverx PathHunter eXpress HEK 293 RELA Nuclear Translocation Assay, 10-plate Kit. (93-0907E1CP0L)
The PathHunter eXpress HEK 293 RELA Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the NFκB pathway. This assay kit enables assessment of ligand (e.g. IL-1α) based pathway activation via detection of resulting RELA nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLink-tagged RELA and nuclear localized EA. Upon pathway activation, induced nuclear translocation of RELA leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mRXFP4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0929E2MCP2M)
The PathHunter mRXFP4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. insulin-like 5, relaxin-3) based activation of mRXFP4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mRXFP4 and EA-tagged β-Arrestin-2. Activation of mRXFP4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ACVRL1/ACVR2 Dimerization Assay, 10pl Kit. (93-0963E3CP0L)
The PathHunter ACVRL1/ACVR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GDF2/BMP9) induced dimerization of ACVRL1/ACVR2 receptors. This product uses EFC technology. The cell line included overexpresses ACVR2 target protein fused to PK alongside ACVRL1 target protein fused to EA. Upon ligand-induced dimerization of ACVRL1/ACVR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HCRTR2 Total GPCR Internalization Assay, 10pl Kit. (93-0730E3CP0L)
The PathHunter HCRTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EGFR/EGFR Dimerization Assay, 10-Plate Kit. (93-0989E3CP5L)
The PathHunter EGFR/EGFR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. EGF) induced dimerization of EGFR/EGFR receptors. This product uses EFC technology. The cell line included overexpresses EGFR target protein fused to PK alongside EGFR target protein fused to EA. Upon ligand-induced dimerization of EGFR/EGFR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR1F U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0699E3CP0L)
The PathHunter HTR1F eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1F GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1F and EA-tagged β-Arrestin-2. Activation of HTR1F stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AVPR1B Activated GPCR Internalization Assay, 2pl Kit. (93-0722E3CP5M)
The PathHunter AVPR1B Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of AVPR1B via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AVPR1B. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AVPR1B-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR1F U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0699E3CP0M)
The PathHunter HTR1F eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1F GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1F and EA-tagged β-Arrestin-2. Activation of HTR1F stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY2 Activated GPCR Internalization Assay, 10pl Kit. (93-0719E3CP5L)
The PathHunter P2RY2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of P2RY2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged P2RY2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting P2RY2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CDC25A Degradation Assay, 10-Plate Kit. (93-0765E3CP0L)
The PathHunter eXpress CDC25A Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the cell cycle checkpoint pathway. This kit enables detection of levels of CDC25a, allowing for assessment of CHK-1-activation-induced CDC25a degradation. This product uses EFC technology. The cell line included overexpresses CDC25a target protein fused to ProLabel. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of CDC25a EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.