General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CHRM5 Activated GPCR Internalization Assay, 2pl Kit. (93-0657E3CP5M)
The PathHunter CHRM5 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress FGFR4-β-Klotho Functional Assay, 10pl Kit. (93-1060E3CP0L)
The PathHunter eXpress FGFR4-β-Klotho Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated FGFR4 and β-Klotho dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FGFR4 and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, β-Klotho. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
FUJIFILM Biosciences ADRA Kit
Amino acid Derivative Reactivity Assay ADRA is an alternative method of skin sensitization test to animal testing ADRA significantly reduces the number of chemical substances used as a reaction reagent by using a novel amino acid derivative having a naphthalene ring capable of high sensitivity detection by ultraviolet rays Also skin sensitization tests are possible even at about 1/100 by conventional methods It is possible to evaluate skin sensitization with high accuracy without causing chemical substances to precipitate in the reaction solution It evaluates whether a chemical substance provokes an allergic reaction in the skin without using animal models Reagent kit for performing ADRA as described in OECD TG 442C
Eurofins Discoverx PathHunter eXpress mCCR3 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0522E2MCP0L)
The PathHunter mCCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL24) based activation of mCCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR3 and EA-tagged β-Arrestin-2. Activation of mCCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MTNR1A CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0951E2CP2L)
The PathHunter MTNR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 2-iodomelatonin) based activation of MTNR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MTNR1A and EA-tagged β-Arrestin-2. Activation of MTNR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress P2RY1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0941E3CP5L)
The PathHunter P2RY1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. ATP) based activation of P2RY1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY1 and EA-tagged β-Arrestin-2. Activation of P2RY1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GHR-JAK1 Functional Assay, 2-Plate Kit. (93-0756E3CP0M)
The PathHunter GHR-JAK1 Functional assay eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting CTKs. This kit assesses GHR activation via detection of JAK1 mediated phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged GHR cytokine receptor, an untagged CTK JAK1 and EA-tagged SH2 domain protein. Upon activation of the cytokine receptor, CTK-based receptor phosphorylation leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Calcein AM Assay Kit (Fluorometric)
Calcein AM Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Bioassay Systems EnzyChrom™ Glucose Assay Kit III. For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.
For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 0.03 to 2 mM (0.54 mg/dL to 36 mg/dL) glucose. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.03 mM.
Abcam D 2 Hydroxyglutarate Assay Kit (Colorimetric)
D 2 Hydroxyglutarate Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Neuraminidase Assay Kit (Fluorometric - Blue)
Neuraminidase Assay Kit (Fluorometric - Blue)
The product is subject to the following: Abcam Restricted Use Statement
Biolegend TotalSeqTM-D1024 anti-human CD
TotalSeq™-D1024 anti-human CD120b [3G7A02]; Isotype: Rat IgG2a, κ; Reactivity: Human; Apps: PG; Size: 10 μg
Cayman Chemical AMPK activator, Purity: ≥95%, CAS: 849727-81-7, 10 mg
An indirect activator of AMPK (EC50 = 11.7 μM); stimulates glucose uptake in L6 myocytes and promotes phosphorylation of ACC, a known target of AMPK; decreases blood glucose concentrations in Zucker diabetic rats, demonstrating its utility in whole animals; inhibits the growth of multiple myeloma cells
Eurofins Discoverx PathHunter eXpressNR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0323E2CP0M)
The PathHunter MLNR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MLNR and EA-tagged β-Arrestin-2. Activation of MLNR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Medchemexpress LLC Kallikrein-5 Mouse 250ug | 250ug
The Kallikrein-5 protein may be involved in desquamation suggesting a role in the process of skin shedding and exfoliation Its association with desquamation suggests that it plays a role in regulating the removal of dead skin cells which is critical for skin homeostasis Kallikrein-5 Protein Mouse (271a a HEK293 His) is the recombinant mouse-derived Kallikrein-5 protein expressed by HEK293 with C-His labeled tag