General Cell Based Assays
Eurofins Discoverx PathHunter eXpress AVPR1A U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0587E3CP0L)
The PathHunter AVPR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR1A and EA-tagged β-Arrestin-2. Activation of AVPR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems QuantiChrom™ Urea Assay Kit II. For quantitative enzymatic determination of urea.
For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 ™L sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD557nm; Samples: Urea in biological samples (e.g. plasma, serum, urine) and food/beverage samples (e.g. milk). Size: 100 tests.
Eurofins Discoverx cAMP Hunter eXpress CXCR3 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0054E2CP2L)
The cAMP Hunter CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR3, a naturally Gi/Go coupled GPCR. Upon activation of CXCR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Genscript Corporation PLAU/uPA active formBiotin
Plasminogen activator urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers PLAU inhibition could suppress tumor growth Collectively PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC
Enzo Life Sciences Cell Counting Kit-8 (100tests)
The Cell Counting Kit-8 is a colorimetric assay kit used to measure cell proliferation and cytotoxicity. It is a ready-to-use solution that does not require radioisotopes and correlates with the [3H]-thymidine incorporation assay. It can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results. CCK-8 has shown to achieve higher sensitivity and stability than MTT, MTS or WST-1. Alternative name: CCKi-8. Applications: Colorimetric detection .
AdipoGen Asprosin human ELISA Kit
Colorimetric Sandwich ELISA Assay. Detects human asprosin. Assay Type Sandwich. Detection Type Colorimetric. Sample Type Cell Culture Supernatant, Plasma, Serum. Range 0.3125 to 20ng/ml. Sensitivity 230pg/ml. Asprosin is a new fasting-induced protein hormone that targets the liver to increase plasma glucose levels. Asprosin is the C-terminal cleavage product of the protein Fibrillin-1. Asprosin is secreted from white adipose tissue and increases hepatic glucose production by using cAMP as a second messenger, leading to activation of protein Kinase A. Reduction of asprosin levels protects against metabolic syndrome-associated hyperinsulinism. Asprosin may act as a circulating hunger signal. Indeed, peripherally injected recombinant asprosin can cross the blood-brain barrier and intracerebroventricular i.c.v. injection of recombinant asprosin stimulated appetite in wild-type mice, indicating a central mechanism of action.
Bioassay Systems EnzyChrom™ Formate Assay Kit. Direct determination of formate concentrations in biological (e.g. urine, blood, etc.) and other samples.
For quantitative determination of formate and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range 0.050 to 5 mM Formate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Urine, Serum etc. Species: All. Procedure: Assay takes approximately 60 min. Kit size: 100 tests. Detection limit: 50 µM.
Eurofins Discoverx PathHunter eXpress TORC1 Nuclear Translocation Assay, 10pl Kit. (93-0833E3CP19L)
The PathHunter eXpress TORC1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the CRTC/CREB pathway. This kit assesses ligand (e.g. forskolin) based pathway activation via detection of resulting TORC1 (CRTC1) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC1 (CRTC1) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPY2R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0212E2CP0L)
The PathHunter NPY2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. peptide YY) based activation of NPY2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPY2R and EA-tagged β-Arrestin-2. Activation of NPY2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF mAb Anti-6His XL665-Conjugate, 5,000 Assay Points
HTRF mAb Anti-6His XL665-Conjugate, 5,000 Assay Points
Lonza Walkersville Sodium Pyruvate GMP 100mL
Sodium Pyruvate GMP Solution is sometimes added to cell culture as an additional source of energy since it is a key intermediate in the production of the high energy intermediate ATP within the cell Its normal concentration in MEM is 1 mM Sodium Pyruvate GMP Solution may help cells that are deficient in the nine enzymatic steps that break glucose down to pyruvate This BioWhittaker Cell Culture Media is produced according to cGMP and labelled For Further Manufacturing (FFM) GMP Products are intended for research or further manufacturing use only The products confirm with cGMP manufacturing compliance expectations for medical devices including 21 CFR Part 820 and ISO 13485 THIS PRODUCT IS NOT INTENDED FOR DIRECT THERAPEUTIC USE IN HUMANS
Eurofins Discoverx PathHunter TrkB-P75 Bioassay Kit, 2-Plate. (93-0530Y3-00075)
The PathHunter TrkB-P75 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. BDNF) induced dimerization of TrkB-P75 receptors. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. Upon ligand-induced dimerization of TrkB-P75, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx HitHunter cAMP Assay for Biologics, 1,000/4,000 dp (96-well/384-well). (90-0075LM10)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or GαI-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 10 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx PathHunter eXpress CHRM1 Activated GPCR Internalization Assay, 10pl Kit. (93-0655E3CP5L)
The PathHunter CHRM1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc Bovine LPO ELISA Kit
This Bovine Lactoperoxidase (LPO) ELISA Kit is a precoated Sandwich ELISA assay designed to detect and quantify the target analyte in milk tears and other biological fluids This product is for Research Use Only