General Cell Based Assays
Eurofins Discoverx PathHunter eXpress mINSRa Functional Assay, 10-Plate Kit. (93-1015E3CP16L)
The PathHunter eXpress mINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated mINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged mINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress DARC CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0548E2CP1M)
The PathHunter DARC eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of DARC GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DARC and EA-tagged β-Arrestin-2. Activation of DARC stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR3 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0626E2CP1M)
The PathHunter GPR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR3 and EA-tagged β-Arrestin-2. Activation of GPR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress LGR6 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0527E2ACP1L)
The PathHunter LGR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of LGR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR6 and EA-tagged β-Arrestin-2. Activation of LGR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Echelon Biosciences Research Labs PI(4,5)P2 DiC16 1 mg
Phosphatidylinositol 4,5-bisphosphate diC16 (PI(4,5)P2 diC16) is a synthetic, purified dipalmitoyl PI(4,5)P2.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to play a central role in a variety of cellular functions. Amongst its many functions, PIP2 is a substrate for Phospholipase C-coupled G-protein pathways involved in intracellular calcium release in a number of tissues and is also a substrate for class I phosphoinositide 3-kinase (PI3-K). This item is non returnable
Abcam Swinholide A, F-actin inhibitor, 50UG
MW 1389.9 Da, Purity >97%. Cell permeable, selective F-actin inhibitor. Sequesters and stabilizes actin dimers. Induces actin depolymerization and apoptosis. .
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery R-PLEX Human CD80/B7-1 ASY
The R-PLEX Human CD80 (B7-1) Assay Kit from Meso Scale Discovery measures CD80 levels in human serum plasma and cell culture supernatants using electrochemiluminescence (ECL) detection It offers high sensitivity broad dynamic range and precision The kit includes a 96-well plate with capture and detection antibodies buffers blocker solutions calibrator and controls Sensitivity is 0 3 pg/mL with an upper quantification limit of 10 000 pg/mL suitable for biomarker discovery and immunology research
Eurofins Discoverx PathHunter eXpress GPR27 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0334E2ACP1M)
The PathHunter GPR27 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR27 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR27 and EA-tagged β-Arrestin-2. Activation of GPR27 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human MIP-1 alpha (CCR1) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human MIP-1 alpha (CCR1) Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure CCR1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress P2RY2 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0628E3CP5M)
The PathHunter P2RY2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY2 and EA-tagged β-Arrestin-2. Activation of P2RY2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EphB2 Functional Assay, 10-Plate Kit. (93-0848E3CP16L)
The PathHunter eXpress EphB2 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB2 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphB2 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human UTR2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human UTR2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure UTR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress CXCR6 HEK 293 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0205E1CP0M)
The PathHunter CXCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL16) based activation of CXCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR6 and EA-tagged β-Arrestin-2. Activation of CXCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CMKLR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0313E2CP0M)
The PathHunter CMKLR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CMKLR1 and EA-tagged β-Arrestin-2. Activation of CMKLR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences ROS-ID Hypoxia/Oxidative stress detection kit (1x500tests)
Specific detection system for simultaneous analysis of hypoxia and oxidative stress using microscopy and flow cytometry. Enzo Life Sciences’ ROS-ID.The Hypoxia (Red) dye takes advantage of the nitroreductase activity present in hypoxic cells by converting the Nitro group to hydroxylamine (NHOH) and amino (NH.The Oxidative Stress Detection Reagent is a non-fluorescent, cell-permeable total ROS detection dye which reacts directly with a wide range of reactive species. The generated fluorescent products can be visualized using a wide-field fluorescence microscope equipped with standard fluorescein (490/525 nm) and Texas Red (596/670 nm) filters, confocal microscopy, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection, HTS. Contents: Hypoxia Red Detection Reagent. Oxidative Stress Detection Reagent (Green). ROS Inducer (Pyocyanin). Hypoxia Inducer (DFO)