General Cell Based Assays
Eurofins Discoverx PathHunter eXpress HTR2C (VGV) U2OS β-Arrestin GPCR Assay, 2pl Kit. (93-0932E3CP19M)
The PathHunter HTR2C(VGV) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C(VGV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VGV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VGV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress AGTRL1 Activated GPCR Internalization Assay, 10pl Kit. (93-0710E3CP0L)
The PathHunter AGTRL1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of AGTRL1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human Phospho-AKT1 (Ser473) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-AKT1 (Ser473) Detection Kit, 50,000 Assay Points
Meso Scale Discovery U-PLEX Human GRO- ASY
The Meso Scale Discovery U-PLEX Human GRO-alpha Assay Kit is a multiplex immunoassay for measuring GRO-alpha in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection for high sensitivity and precision Requiring 50 L or less sample volume it can be automated on MSD platforms The kit includes a 96-well plate with capture and detection antibodies calibrators and controls for standard curve generation and assay validation It allows simultaneous measurement of GRO-alpha and other biomarkers with high reproducibility
Cell Biolabs Inc DAG (Diacylglyercol) Assay Kit, 100 assays
Diacylglycerols (DAG) are key intermediates in the biosynthesis of many cellular lipids and play a fundamental role in biochemical signaling. Cell Biolabs’ DAG Assay Kit simple 96-well fluorometric assay that measures diacylglycerol content in samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG samples, yielding phosphatidic acid. Next, a lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Finally, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide which reacts with the kit’s fluorometric probe. The kit contains a DAG Standard and has a detection sensitivity limit of 15 µM. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. Note each sample replicate requires 2 assays, one of which is a negative control to be subtracted as background.
Eurofins Discoverx PathHunter eXpress CSF2RB/CSF2RA Dimerization Assay, 10pl Kit. (93-1078E3CP22L)
The PathHunter CSF2RB/CSF2RA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. GM-CSF) induced dimerization of CSF2RB/CSF2RA receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside CSF2RA target protein fused to EA. Upon ligand-induced dimerization of CSF2RB/CSF2RA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems EnzyChrom™ Nitric Oxide Synthase Assay Kit. For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor.
For quantitative determination of nitric oxide synthase (NOS) activity and screen for NOS inhibitor. Key Features: Sensitive and accurate. Detection range 0.25 - 25 U/L in 96-well plate. Rapid and reliable. Can be completed in 40 min if reduction of NO3- to NO2- is performed at 60°C. Method: OD540nm. Samples: Biological. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.25 U/L.
Eurofins Discoverx PathHunter PTH Bioassay Kit, 2-Plate. (93-0315Y2-00047)
The PathHunter PTH Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses PTH based activation of PTHR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTHR and EA-tagged β-Arrestin-2. Activation of PTHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam Transketolase Activity Assay Kit (Fluorometric)
Transketolase Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress IL4R/IL13RA1 Dimerization Assay, U2OS, 10pl Kit. (93-1000E3CP5L)
The PathHunter IL4R/IL13RA1 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-13) induced dimerization of IL4R/IL13RA1 receptors. This product uses EFC technology. The cell line included overexpresses IL4R target protein fused to PK alongside IL13RA1 target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL13RA1, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human HSP27/HspB1 ASY
The R-PLEX Human HSP27 (HSPB1) Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay that measures human HSP27 protein levels in serum plasma cell culture supernatants cell and tissue lysates and other biological samples This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker solutions calibrator and controls This assay allows for the quantitative measurement of HSP27 levels with high precision and reproducibility
ACROBiosystems Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is expressed from Human, 500 tests
Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is expressed from Human
Eurofins Discoverx PathHunter eXpress GPR92 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0372E2CP6M)
The PathHunter GPR92 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. LPA) based activation of GPR92 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR92 and EA-tagged β-Arrestin-2. Activation of GPR92 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Lipid Assay Kit (neutral lipids)
Lipid Assay Kit (neutral lipids)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress CXCR7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0248E2CP2L)
The PathHunter CXCR7 (CMKOR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of CXCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR7 and EA-tagged β-Arrestin-2. Activation of CXCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.