General Cell Based Assays
Abcam Phalloidin-iFluor 405 Reagent
Phalloidin-iFluor 405 Reagent
The product is subject to the following: Abcam Restricted Use Statement
Electron Microscopy Sciences Flim Sleeve 135 mm/20 exp with Frosted Open Flap
Polyguard Polypropylene film sleeves are the finest archival quality material, and are guaranteed to give you ultimate protection for your most important film negatives. Polyguard is 100% acid free, as well as being free from optical distortion. It is resistant to water, grease, oil, alkalies, and organic solvents. Polyguard is durable, yet pliable and gentle to the film and will not become brittle, crack, or abrade the film.
Type: Frosted Open Flap
Cell Biolabs Inc MTT CELL PROLIFERATION ASSAY
CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring cell proliferation. Reagents are sufficient for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.
Meso Scale Discovery R-PLEX Human AXL/UFO ASY
The R-PLEX Human AXL UFO Assay from Meso Scale Diagnostics is a multiplex immunoassay kit that allows for the simultaneous quantitative measurement of AXL in human serum plasma and cell culture supernatants It utilizes Meso Scale Diagnostics electrochemiluminescent detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator diluent and lyophilized calibrator It can test up to 80 samples in duplicate and has a testing time of 3 5 hours
Selleck Chemical LLC Smoothened Agonist (SAG) HCl 2mg 2095432-58-7
Smoothened Agonist (SAG) HCl is a cell-permeable Smoothened (Smo) agonist with EC50 of 3 nM in Shh-LIGHT2 cells. *For Research & Development use only. Product is not intended for drug, household, or human consumption.
Abcam DNMT3B Assay Kit.
DNMT3B Assay Kit.
The product is subject to the following: Abcam Restricted Use Statement
Dojindo Molecular Technologies Inc SOD Assay Kit-WST (500 TESTS) is a colorimetric microplate and low background noise measurement which measures 100% inhibition by SOD.
Superoxide dismutase (SOD) is a crucial antioxidative enzyme, which catalyzes the dismutation of the superoxide anion (O2-) into hydrogen peroxide and molecular oxygen. In order to determine SOD activity, several direct and indirect methods have been developed. Among these methods, the indirect method using nitroblue tetrazolium (NBT) is commonly used due to the convenience and ease to use. However, using the NBT method yields poor water solubility of the formazan dye and the interaction with the reduced form of xanthine oxidase. SOD Assay Kit-WST allows for very convenient SOD assays by utilizing Dojindo's highly water-soluble tetrazolium salt, WST-1, producing a water-soluble formazan dye upon reduction with the superoxide anion. The rate of the reduction of WST-1 with O2- is linearly related to the xanthine oxidase (XO) activity depending on SOD. Therefore, the IC50 (50% inhibition activity of SOD or SOD-like materials) can be determined by the colorimetric method.
Cayman Chemical PhagocytosIs asy KItIgG P 1ea
Cayman’s Phagocytosis Assay Kit (IgG PE) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis at the single-cell level. In addition, the flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-PE Complex for up to 750 samples.
Bellbrook Labs TRANSCREENER ADP2 TR-FRET RED ASSAY
Transcreener ADP2 TR-FRET Red Assay (1,000 Assay, 384 Well)
Molecular Devices Neurotransmitter Uptake Assay, Explorer, Homogeneous, 96/384-Well
Molecular Devices' Neurotransmitter Transporter Uptake Assay Explorer Kit provides a homogeneous, fluorescence-based method to measure dopamine, norepinephrine, and serotonin transporter (DAT, NET, SERT) uptake activity in live cells. The no-wash format supports kinetic and endpoint reads and is optimized for high-throughput screening and for use with transfected or endogenously expressing cells.
- Homogeneous no-wash assay format.
- Fluorescent substrate for DAT, NET, and SERT uptake.
- Five lyophilized reagent vials, each sufficient for one 96- or 384-well plate.
- Compatible with bottom-read fluorescence microplate readers and kinetic read modes.
- Store reagents at -20°C; dye solution stable up to eight hours at room temperature or up to five days when aliquoted and frozen.
Molecular Devices FLIPR Calcium 6 Assay Kit, 10-Plate Explorer
The Molecular Devices FLIPR Calcium 6 Explorer Kit provides reagents for fluorescent intracellular calcium flux assays optimized for FLIPR-compatible high-throughput readers. The kit supplies enough reagent for ten assay plates and is engineered to deliver a high signal-to-background window for low-signal targets such as endogenous, primary, or stem cell assays.
- Explorer kit format supplies reagents for 10 assay plates.
- Compatible with 96-well, 384-well, and 1536-well plate formats.
- Includes 10 vials of Component A and 1 bottle of dilution/assay buffer (Component B).
- Optimized calcium indicator with high quantum yield for strong signal-to-background.
- Suitable for FLIPR-compatible plate readers and high-throughput calcium flux screening.
Molecular Devices Fluorescent Assay Kit, cAMP, 96-Well, Bulk
The CatchPoint cAMP Fluorescent Assay Kit is a 96-well competitive immunoassay for quantitative measurement of intracellular or biochemical cAMP levels in cell-based and enzymatic assays. It provides high-affinity reagents and pre-coated microplates optimized for sensitive fluorescence detection.
- Format: 96-well bulk kit with reagents for ten 96-well plates.
- Target analyte: 3',5'-cyclic adenosine monophosphate (cAMP).
- Detection: fluorescent competitive immunoassay, 120 μL total assay volume per well.
- Contents: pre-coated black 96-well plates, rabbit anti-cAMP antibody, HRP-cAMP conjugate, cAMP calibrator, substrate, assay buffer, wash concentrate, cell lysis buffer.
- Incubation: 2 hours for antibody/conjugate incubation; substrate signal readable from 10 minutes up to 24 hours.
- Storage: store components at 4°C; reconstituted reagents stable for about 3 weeks at 4°C.
Agilent Technologies Seahorse XF Glycolytic Rate Assay Kit
Seahorse XF Glycolytic Rate Assay Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of rotenone/antimycin A and 2-deoxy-D-glucose. For use with XFe/XF96 and XFe24 analyzers. Not compatible with XF24 and XF24-3 analyzers.
Abcam Lipid Peroxidation Assay Kit (Cell-based)
Lipid Peroxidation Assay Kit (Cell-based)
The product is subject to the following: Abcam Restricted Use Statement
Molecular Devices Calcium Assay Kit, Fura-2 QBT, Homogeneous, No-Wash
The Fura-2 QBT Calcium sample kit is a homogeneous, mix-and-read assay designed to measure intracellular calcium mobilization using the ratiometric dye Fura-2. Proprietary quench/masking chemistry enables a no-wash protocol to reduce steps and minimize assay artifacts.
- Homogeneous mix-and-read assay for calcium mobilization.
- No-wash protocol to reduce assay steps and variability.
- Ratiometric Fura-2 dye provides excitation at 340/380 nm, emission at 510 nm.
- Sample kit format contains two plates worth of reagent.
- Compatible with plate-based fluorescence readers and FLIPR systems.
- Minimizes effects of uneven dye loading and dye leakage on results.