General Cell Based Assays
Cayman Chemical 6-alkyn NAD sodIum salt 700ug
A clickable form of NAD+; has been used in conjunction with fluorescent tags in the detection of PARP protein substrates
TRIBIOSCIENCE INC Mitochondrial Complex III Activity Colorimetric Assay 100 Tests
The activity of Complex III is directly measured through the increase of reduction of cytochrome c at absorbance of 550nm. This kit uses cytochrome c as substrate. The reduced cytochrome c is determined by recording the change in absorbance (A550). Applications Complex III Activity.
TRIBIOSCIENCE INC Glutamate Quantification Colorimetric Assay 100 Tests
Tribioscience Glutamate Quantification Colorimetric Assay is based on a dehydrogenase coupled reaction converting chromophore to a colored formazan, which can be measured at OD 460 nm. The generated signal is proportional to the content of glutamate in the samples. The kit provides the easiest and most accurate approach to measuring glutamate from a variety of samples. Applications - Specifically detect glutamate from cell and biological tissues.
American Research Products Inc CREB (Phospho-Ser121) Transcription Factor Activity Assay
CREB (Phospho-Ser121) Transcription Factor Activity Assay
Cell Biolabs Inc Glycogen Assay Kit (Fluorometric), 100 assays
Cell Biolabs’ Glycogen Assay Kit (Fluorometric) is a simple assay that measures the amount of total glycogen present in biological samples in a 96-well fluorescence plate reader. Glycogen is broken down into glucose monomers by amyloglucosidase first, glucose is then oxidized by glucose oxidase into D-gluconic acid and hydrogen peroxide. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe in a standard 96-well colorimetric plate reader. Sample glycogen concentrations are determined by comparison with a known glycogen standard. The kit has a detection sensitivity limit of 120 nM glycogen. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, glycogen standards, and unknown samples. Note each sample replicate requires 2 assays, one of which is a negative control to be subtracted as background.
ION BIOSCIENCES Brilliant Thallium Gold Assay Flex kit - 10 plates
ION Biosciences' Brilliant Thallium Gold Assay Flex kit is a total assay solution for multi-well plate-based, high-throughput measurements of Tl+ flux through K+, Na+, non-selective cation channels, and some Na+ or K+ transporters. The kit includes our proprietary Thallos Gold indicator (Ex/Em: 530/546 nm), which is responsive to intracellular Tl+ dynamics. Thallos Gold’s spectral properties allow for multiplexing with GFP-expressing cells or other green-fluorescent indicators, and will help mitigate false positives caused by auto-fluorescent compounds within a library. The ION Biosciences Brilliant Thallium Gold Assay is also useful for a wide range of effectors of ion channels and transporters including G protein-coupled receptors, lipid kinases and protein kinases. 10\ plate kit.
ION BIOSCIENCES Thallium-free GIRK Potassium Channel Assay Flex kit - 10 plates
ION Biosciences' Thallium-free GIRK Potassium Channel Assay Flex kit is the first fluorescence-based potassium channel solution that directly measures potassium flux, enabling you to move away from thallium-containing products. This product features the potassium-sensitive dye, IPG-1 AM, our lowest affinity potassium indicator to measure changes in intracellular potassium concentration. This kit is optimized for GIRK (Kir3.x) screening, and can be used to identify activators and inhibitors of GIRK channels. Traditional approaches for measuring GIRK activity such as patch clamp, FRET, or thallium flux assays can be labor-intensive, low-throughput, or reliant on toxic surrogate ions. To overcome these limitations, ION Biosciences built the Thallium-Free GIRK Potassium Channel Assay featuring ION Potassium Green-1 AM (IPG-1 AM), a membrane-permeable, potassium-sensitive, fluorescent indicator that directly measures potassium flux. 10 plate kit.
ETON BIOSCIENCE INC L-LACTATE ASSAY KIT 5ML
Lactate dehydrogenase converts lactate and NAD+ into pyruvate and NADH. The Lactate Assay Kit is based on the reduction of the terazolium salt INT in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm.
American Research Products Inc TFE3 Transcription Factor Activity Assay
TFE3 Transcription Factor Activity Assay